Volatile profiles of toxigenic and non-toxigenic Aspergillus flavus using SPME for solid phase extraction.

Volatile profiles of toxigenic and non-toxigenic Aspergillus flavus using SPME for solid phase extraction.

Annals of agricultural and environmental medicine : AAEM (2010-12-29)

Anthony J De Lucca, Stephen M Boué, Carol H Carter-Wientjes, John M Bland, Deepak Bhatnagar, Thomas E Cleveland

ABSTRACT

Toxigenic and atoxigenic strains of Aspergillus flavus were grown on potato dextrose agar (PDA) and wetted (23% moisture) sterile, cracked corn for 14 and 21 days, respectively. Volatile compounds produced by A. flavus, as well as those present in the PDA controls and sterile cracked maize, were collected using solid-phase micro-extraction (SPME) and identified by gas chromatography/mass spectrometry. Results show that growth substrate had a major impact on the number and type of volatiles detected. Growth on sterile cracked maize produced many more volatiles than did potato dextrose agar. There were also differences observed in the type of volatiles produced between toxigenic and non-toxigenic isolates, as well as between isolates of the same toxigenic grouping.

MATERIALS

Product Number

Brand

Product Description

57284-U

Supelco

SPME Fiber Assembly Kit, Assortment, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 4

57285-U

Supelco

SPME Fiber Assembly Kit, Assortment #1, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3

57286-U

Supelco

SPME Fiber Assembly Kit, Assortment #2, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3

57287-U

Supelco

SPME Fiber Assembly Kit, Assortment #4, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3

57320-U

Supelco

SPME Fiber Assembly Kit, Assortment #2, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3

57551-U

Supelco

SPME Fiber Assembly Kit, Assortment, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 4

57325-U

Supelco

SPME Fiber Assembly Kit, Assortment #4, Fused Silica (1 cm), size 24 ga, Autosampler, pk of 3

57550-U

Supelco

SPME Fiber Assembly Kit, Assortment, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 4

57306

Supelco

SPME Fiber Assembly Kit, Assortment #1, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3

57321-U

Supelco

SPME Fiber Assembly Kit, Assortment #2, Fused Silica (1 cm), size 24 ga, Autosampler, pk of 3

57324-U

Supelco

SPME Fiber Assembly Kit, Assortment #4, Fused Silica (1 cm), size 24 ga, Manual Holder, pk of 3

57307

Supelco

SPME Fiber Assembly Kit, Assortment #1, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3

57362-U

Supelco

SPME Fiber Assembly Kit, Assortment #5, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 4

57294-U

Supelco

SPME Fiber Assembly, 85 μm Polyacrylate, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, white hub

57328-U

Supelco

SPME Fiber Assembly, 50/30µm DVB/CAR/PDMS, StableFlex (1cm), needle size 24 ga, Manual Holder, pk of 3, plain gray hub

57335-U

Supelco

SPME Fiber Assembly, 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, light blue hub

57345-U

Supelco

SPME Fiber Assembly, 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, blue hub

57357-U

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SPME Sampling Stand, for use with 15 mL vials

57299-U

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SPME Fiber Assembly, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 23 ga, Autosampler, pk of 3, notched gray hub

57291-U

Supelco

SPME Fiber Assembly, 7 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, green hub

57293-U

Supelco

SPME Fiber Assembly, 65 μm PDMS/DVB, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, pink hub

57295-U

Supelco

SPME Fiber Assembly, 85 μm CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, light blue hub

57298-U

Supelco

SPME Fiber Assembly, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, plain gray hub

57906-U

Supelco

SPME Fiber Assembly, 85 μm CAR/PDMS, Metal Alloy (1 cm), needle size 23 ga, Autosampler, pk of 1, light blue hub

57912-U

Supelco

SPME Fiber Assembly, 50/30µm DVB/CAR/PDMS, Metal Alloy (1cm), needle size 23 ga, Autosampler, pk of, plain gray hub

57902-U

Supelco

SPME Fiber Assembly, 65 μm PDMS/DVB, Metal Alloy (1 cm), needle size 23 ga, Autosampler, pk of 1, pink hub

57311

Supelco

SPME Fiber Assembly, 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, blue hub

57302

Supelco

SPME Fiber Assembly, 7 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, green hub

57326-U

Supelco

SPME Fiber Assembly, 65 μm PDMS/DVB, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, pink hub

57303

Supelco

SPME Fiber Assembly, 7 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, green hub