The volume of this product is lot specific and dependent upon the protein content and enzymatic activity. Volume may be determined using the specific activity value and mg protein/mL value reported in the Certificate of Analysis. For example, if the end-user has 500KU pack size and the specific activity is reported as 700 units/mg, while the concentration is 20.1 mg/ml. The volume would equate to roughly 35.52 mL. Please see the link below to review a sample or lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/mm/cx2335#product-documentation
A3403
α-Amilasi
Type XII-A, saline solution, ≥500 units/mg protein (biuret)
Sinonimo/i:
1,4-α-D-Glucan-glucanoidrolasi
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500000 UNITS
140,00 €
1000000 UNITS
257,00 €
5000000 UNITS
965,00 €
140,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Informazioni su questo articolo
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Vai a
Servizio Tecnico
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Permettici di aiutartiTipo
Type XII-A
Livello qualitativo
Stato
saline solution
Attività specifica
≥500 units/mg protein (biuret)
PM
57.6 kDa
Caratteristiche più verdi
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
Categoria alternativa più verde
Temperatura di conservazione
2-8°C
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Questo articolo | A4582 | A4551 | A3306 |
|---|---|---|---|
| specific activity ≥500 units/mg protein (biuret) | specific activity - | specific activity 500-1,500 units/mg protein | specific activity 20000—60000 U/mL |
| form saline solution | form liquid | form lyophilized powder | form solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt 57.6 kDa | mol wt - | mol wt 58-62 kDa | mol wt - |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 300 |
| type Type XII-A | type - | type - | type - |
Descrizione generale
α-Amylase is a member of the endo-amylase family. Its molecular weight is 57.6 kDa. α-Amylase is composed of three functional domains namely A, B and C. Domain A is a barrel-shaped (β/α)8 structure and is the largest domain. Domain B is linked to domain A via disulfide bond and is present between domain A and C. Domain C is composed of β-sheets and is connected to domain A via a polypeptide chain.[1]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
Applicazioni
α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A3403 is from Bacillus licheniformis and is type XII-A. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis [2].
Azioni biochim/fisiol
α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose.
Stato fisico
Aqueous solution containing sodium chloride and sucrose
Altre note
One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.
Note legali
A product of Novozyme Corp.
Termamyl is a registered trademark of Novozymes Corp.
Esclusione di responsabilità
Reported to be heat stable at temperatures as high as ∼90 °C.
used together
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Protocolli
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
Contenuto correlato
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What is the volume for the A3403-500KU
1 risposta-
Utile?
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What is the optimal conditions (pH and temperature) for this enzyme to degrade starch? Will it deactivate after heating to 100C?
1 risposta-
This product has an optimum pH range of 5–9 and will maintain 98% of activity after 60 minutes at pH 6.2 at 85°C. This material is tested pH 6.9 at boiling temperature for 15 minutes. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/297/885/a3403pis.pdfUtile?
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Dear Sir or Madam Could you tell me exactly in what solution the a-amylase is provided? Unfortunately only a "saline sucrose solution" is stated. I would need the exact composition in order to prepare a negative control in my assay. kind regards
1 risposta-
All that we can disclose is that this product is supplied as an aqueous solution containing sodium chloride and sucrose with >=10 mg/mL protein.
Utile?
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