This product is intended for research use only and is not suitable for human or veterinary use. Currently, a food grade product is not available.
P4860
Proteasi
≥2.4 U/g
Sinonimo/i:
Proteinasi, Subtilisina A
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50 ML
165,00 €
250 ML
369,00 €
165,00 €
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Permettici di aiutartiStato
aqueous solution
Livello qualitativo
Attività specifica
≥2.4 U/g
Temperatura di conservazione
2-8°C
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Categorie correlate
1 of 4
Questo articolo | P5380 | P3910 | P8038 |
|---|---|---|---|
| specific activity ≥2.4 U/g | specific activity 7-15 units/mg solid | specific activity - | specific activity ≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid |
| form aqueous solution | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 300 |
Descrizione generale
Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.[1]
Applicazioni
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations.
Azioni biochim/fisiol
Alcalase is an endo-protease of the serine type. It has a broad substrate specificity and can hydrolyze most peptide bonds within a protein molecule. It is active between pH 6.5 and 8.5 and has an optimum temperature of 60 °C. The enzyme is is used in detergent formulations to remove protein-based stains.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .
Risultati analitici
Activity expressed in Anson Units
Note legali
A product of Novozyme Corp.
Alcalase is a registered trademark of Novozymes Corp.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
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Neurolysin (Nln) is a recently recognized endogenous mechanism functioning to preserve the brain from ischemic injury. To further understand the pathophysiological function of this peptidase in stroke and other neurologic disorders, the present study was designed to identify small molecule
Andrew R Hirst et al.
Nature chemistry, 2(12), 1089-1094 (2010-11-26)
Supramolecular gels, which demonstrate tunable functionalities, have attracted much interest in a range of areas, including healthcare, environmental protection and energy-related technologies. Preparing these materials in a reliable manner is challenging, with an increased level of kinetic defects observed at
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Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff worldwide. However, no reports on the antitumor peptides from such a microalgae are available in the literature. In this study, using low-temperature high-pressure extraction, enzymatic hydrolysis, ion
Catherine Cesa-Luna et al.
PloS one, 15(10), e0240545-e0240545 (2020-10-16)
The bacterial strain, EMM-1, was isolated from the rhizosphere of red maize ("Rojo Criollo") and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production
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Applied microbiology and biotechnology, 87(6), 2087-2096 (2010-06-04)
Microbial transglutaminase (MTG) has been used extensively in academic research and the food industries through its cross-linking or posttranslational modification of proteins. Two enzyme engineering approaches were applied to improve MTG activity. One is a novel method of rational mutagenesis
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Is this a food grade product?
1 risposta-
Utile?
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What is the density of the P4860 enzyme?
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This item is a product of Novozymes Corp. The supplier reports a density of 1.17g/ml at 20 degrees C.
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What is optimal temperature and ph for this enzyme?
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According to the manufacturer’s (Novozymes Corp.) Product Sheet, by analyzing Alcalase at various pH values using a modified Anson/haemoglobin method, its optimum activity has been found to be at pH between 6.5 and 8.5; its temperature optimum is around 60°C, but could vary between 55°C and 70°C, depending on the type of substrate.
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How many mg of enzyme/mL of solution are there?
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The density of the solution is 1.17 g/mL at 20 °C.
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Can this be used for production of fish protein hudrolysate? Also what is the difference between this enzymeand Alcalse 2.4l?
1 risposta-
This product is identical to Alcalase 2.4L. This material has not been tested for use in fish. A search of the literature shows a number of publications related to the use of this enzyme in animal protein production. However, a specific reference for use in fish was not found. Please see the links below to review articles that may be of interest:
Chicken meat
https://ift.onlinelibrary.wiley.com/doi/10.1111/j.1750-3841.2008.00765.xFish scales
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063752/Utile?
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What is the extinction coefficient?
1 risposta-
The extinction coefficient is not determined for this product.
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What is the extinction coefficient for this enzyme?
1 risposta-
The extinction coefficient for this enzyme is not determined.
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What is the molecular weight?
1 risposta-
The molecular weight of Protease from Bacillus licheniformis (Product No. P4860) is approximately 27 kDa.
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What is the concentration of the product, and how can the conversion from units/g on the COA to units/ml be performed?
1 risposta-
The density of the Novozymes product, as indicated in the SDS sheet, is 1.17g/ml. By multiplying 1.17 g/ml (density) by 50 ml (pack size), 58.5 g of the solution is obtained. Further multiplication with 2.55 unit/g (lot activity) results in 149.175 or 149.2 units. Dividing 149.2 units by 50ml gives 2.984 units/ml.
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What is the optimal pH for the use of enzyme P4860?
1 risposta-
According to the manufacturer Novozyme, the optimal pH for the use of product P4860 is approximately pH 9.
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