Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference: J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.
T1021
Trypsin inhibitor
powder, suitable for isoelectric focusing (IEF)
Sinonimo/i:
SBTI
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30,80 €
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Permettici di aiutartiNome del prodotto
Inibitore della tripsina, Isoelectric focusing marker, pI 4.6
biological source
Glycine max (soybean)
form
powder
mol wt
20,100 Da
technique(s)
isoelectric focusing (IEF): suitable
pI
4.6
solubility
balanced salt solution: 1 mg/mL
concentrate: >10 mg/mL, hazy, amber-yellow
phosphate buffer: 10 mg/mL
water: 10 mg/mL
serum-free medium: soluble
shipped in
ambient
storage temp.
−20°C
Quality Level
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
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Questo articolo | T9767 | 93620 | T6414 |
|---|---|---|---|
| biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| form powder | form powder | form lyophilized powder | form solution |
| shipped in ambient | shipped in ambient | shipped in wet ice | shipped in dry ice |
| solubility balanced salt solution: 1 mg/mL, phosphate buffer: 10 mg/mL, serum-free medium: soluble, concentrate: >10 mg/mL, hazy, amber-yellow, water: 10 mg/mL | solubility balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, phosphate buffer: 10 mg/mL, water: 10 mg/mL, serum-free medium: soluble | solubility 0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless | solubility balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, phosphate buffer: 10 mg/mL, water: 10 mg/mL, serum-free medium: soluble |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
Biochem/physiol Actions
Questo inibitore agisce nei confronti della tripsina e, in misura minore, della chimotripsina e della plasmina. Inibisce anche le proteasi con meccanismi simili alla tripsina, la callicreina plasmatica e il fattore X della coagulazione. L′inibitore della tripsina non è attivo nei confronti delle metalloproteasi, della callicreina tissutale, delle proteasi acide o delle tioproteasi. Questo inibitore agisce formando un complesso stechiometrico 1:1 con il sito attivo della proteasi e quindi scindendo un singolo legame arginina-isoleucina sull′inibitore. L′inibizione è reversibile e dipendente dal pH.
Other Notes
Una unità di tripsina = A253 di 0,001 per minuto con N-alfa-benzoil-L-arginina etil estere (BAEE) come substrato a pH 7,6 e 25 °C
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.
Preparation Note
L′inibitore della tripsina è solubile in acqua e tamponi fosfato 10 mg/mL. È solubile in soluzioni saline bilanciate a 1 mg/mL e in terreni privi di siero. Soluzioni concentrate oltre 10 mg/mL possono essere leggermente torbide e di colore da giallo ad ambrato. Dopo la tripsinizzazione delle cellule, risospendere in 1 mL di soluzione di inibitore della tripsina a 1 mg/mL per ogni mL di soluzione di tripsina usata per la dissociazione. La sospensione cellulare deve quindi essere centrifugata a 1.000 rpm per formare un pellet cellulare.
Le soluzioni possono mantenersi attive se conservate a 2-8 °C a breve termine. Le soluzioni sono stabili in aliquote congelate a -20 °C.
Le soluzioni possono mantenersi attive se conservate a 2-8 °C a breve termine. Le soluzioni sono stabili in aliquote congelate a -20 °C.
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Protocolli
Natural trypsin inhibitors (serpins) regulate protein activation and catabolism by inhibiting serine proteases in vivo.
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Instructions
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What is the molecular weight and amino acid sequence of trypsin inhibitor from Glycine max (soybean)?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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