T2327

Trypsin inhibitor

lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

• Sinonimo/i: Kunitz Inhibitor
• Numero CAS: 9035-81-8
• UNSPSC Code: 12352204
• NACRES: NA.77
• EC Number: 232-906-9
• MDL number: MFCD00082102

Proprietà

• Nome del prodotto: Trypsin Inhibitor from Glycine max (soybean), BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)
• biological source: Glycine max (soybean)
• product line: BioUltra
• assay: ≥95% (Kunitz inhibitor, SDS-PAGE)
• form: lyophilized powder
• storage temp.: 2-8°C
• Quality Level: 200

Descrizione

Analysis Note

One mg will inhibit ≥1.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Application

Trypsin Inhibitor from Glycine max (soybean) has been used:

• as a standard protein to measure the amount of endogenous trypsin inhibitor present in midgut lysate (M1) of Riptortus pedestris[1]
• as a standard to compare the trypsin inhibitory activity of the purified protein[2]
• to monitor the trypsin inhibitory activity by fractionating in MonoS cation exchange chromatography[3]
• as an trypsin inhibitor[4]

Biochem/physiol Actions

Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x10⁸.

Trypsin Inhibitor from Glycine max (soybean) binds with the active site of trypsin enzyme, in a competitive inhibition manner.[5]

General description

Trypsin Inhibitor from Glycine max (soybean) also known as Kunitz trypsin inhibitor is a 21 kDa protein with a single trypsin binding reactive site.[3]

Other Notes

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

View more information on Trypsin Inhibitor.

Preparation Note

Further purification of T9128 yielding an electrophoretically pure Kunitz inhibitor with increased activity.

Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.

Informazioni sulla sicurezza

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Classe di stoccaggio: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)