HPLC Analysis of Rapamycin and 32-Desmethoxyrapamycin on Discovery® HS C18
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CONDITIONS
column
Discovery HS C18, 15 cm × 4.6 mm ID, 3μm particles (568522-U)
mobile phase
[A] 10 mM ammonium acetate, pH unadjusted: [B] acetonitrile (25:75, v/v, A:B)
flow rate
1.5 mL/min
column temp.
60 °C
detector
UV, 268 nm
injection
10 μg/mL
Descrizione
Note legali
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany