1.16141

Sucrose (Saccharose) Test

reflectometric, 0.25-2.50 g/L (sucrose), for use with REFLECTOQUANT^®

Properties

• description: quantitative
• Quality Level: 100
• analyte chemical class(es): sugars
• reaction suitability: reaction type: enzymatic reaction
• measuring range: 0.25-2.50 g/L (sucrose)
• compatibility: RQflex^® 10 Plus; RQflex^® 10; RQflex^® 20; for use with REFLECTOQUANT^®
• detection method: reflectometric
• storage temp.: 2-8°C

Description

General description

Method: reflectometric with test strips and reagent 0.25 - 2.50 g/l Reflectoquant^®

The Reflectoquant Sucrose test is suitable for determination of sucrose in beverages, e. g. fruit/vegetable juice and refreshment drinks after appropriate sample pretreatment or dilution. Sucrose (saccharose) is cleaved by sucrose phosphorylase into fructose and glucose-1-phosphate. The latter is converted by phosphoglucomutase into glucose-6-phosphate. This is in turn oxidized by NAD under the catalytic effect of glucose-6-phosphate dehydrogenase to gluconate-6-phosphate. In the presence of diaphorase the NADH formed in the process reduces a tetrazolium salt to a blue formazan that is determined reflectometrically on the RQflex. For the determination of only glucose please refer to our MQuant or Reflectoquant Glucose tests.

Packaging

50, 50 tests

Linkage

Replaces: 1161410007; 1.16141.0007

Analysis Note

function control: entspricht
Assay
- standard 1:
0.26 - 0.34 mg/l
- standard 2: 1.12 - 1.38 mg/l
- standard 3: 1.80 - 2.20 mg/l

Legal Information

REFLECTOQUANT is a registered trademark of Merck KGaA, Darmstadt, Germany

RQFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10-13 - German Storage Class 10 to 13