Merck
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P7059

Sigma-Aldrich

Phosphate Buffered Saline

10× PBS for Western blots and IP

Synonym(s):
Phosphate buffered saline, PBS
MDL number:
eCl@ss:
32129211
NACRES:
NA.25

Quality Level

grade

for molecular biology

sterility

0.2 μm filtered

form

liquid

pH

7.2-7.6(1x solution)

storage temp.

room temp

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General description

Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Application

Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.

Components

10x PBS contains:
  • 30mM sodium phosphate
  • 1.5 M sodium chloride
  • 10.5 mM potassium phosphate

Preparation Note

Dilute 10x PBS to a 1x working solution using molecular biology grade water.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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