100500C

E. coli Extract Total

Avanti Polar Lipids 100500C

• Synonym(s): E. coli Total Lipid Extract
• CAS Number: 1240502-50-4

Properties

• form: chloroform solution
• packaging: pkg of 2 × 20 mL (100500C-1g); pkg of 1 × 4 mL (100500C-100mg); pkg of 5 × 4 mL (100500C-500mg)
• manufacturer/tradename: Avanti Polar Lipids 100500C
• concentration: 25 mg/mL (100500C-100mg); 25 mg/mL (100500C-1g); 25 mg/mL (100500C-500mg)
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.

Application

E. coli Extract Total is suitable:

• for solubilization of His6-tagged wtABCG2 and its steroid-binding element (SBE) mutant variants
• to prepare E. coli liposomes for liposomal content release assay
• for the determination of the phospholipid types bound to purified AdiC in DDM (n-dodecyl-β-D-maltopyranoside) and OM (n-octyl-β-D-maltopyranoside)

E. coli extract total has been used for the preparation of liposomes. It has also been used to characterize the ternary complexes obtained after purification of membrane proteins in different detergents.

E. coli Extract Total has been used:

• in the preparation of small unilamellar vesicles (SUVs)
• as a reference standard in electrospray ionization tandem mass spectrometry (ESI-MS/MS)
• in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies

Packaging

100, 500 mg

1 g

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100500C-1g)

5 mL Clear Glass Sealed Ampule (100500C-100mg)

5 mL Clear Glass Sealed Ampule (100500C-500mg)

Biochem/physiol Actions

The E.coli lipid extract contains phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. The E. coli lipid extract serves as a standard for profiling phospholipids, aids in the identification and taxonomic grouping of any bacterial sample. It is also used for preparing supported lipid bilayers.

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H302 - H315 - H319 - H331 - H336 - H351 - H361d - H372 - H412
• Precautionary Statements: P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
• Target Organs: Central nervous system
• Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 3
• Flash Point(F): does not flash
• Flash Point(C): does not flash