Merck
All Photos(1)

1.09684

Supelco

Formamide

for analysis EMSURE®

Synonym(s):
Amide C1, Formic amide, Formic acid amide, Methane amide
Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
Beilstein:
505995
MDL number:
EC Index Number:
200-842-0

Quality Level

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

product line

EMSURE®

assay

≥99.2% (N)

form

liquid

autoignition temp.

932 °F

potency

5325 mg/kg LD50, oral (Rat)
17000 mg/kg LD50, skin (Rabbit)

expl. lim.

2.7-19.0 % (v/v)

technique(s)

hybridization: suitable

impurities

≤0.005% Sulfated ash (600 °C)
≤0.02% Formic acid
≤0.1% Ammonium Formate
≤0.1% Water (Karl Fischer)
≤1 ppm Cd (Cadmium)
≤1 ppm Chloride (Cl)
≤1 ppm Cu (Copper)

color

clear
APHA: ≤10

refractive index

n20/D 1.447 (lit.)

pH

8-10 (20 °C, 200 g/L in H2O)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

transition temp

flash point 175 °C

density

1.134 g/mL at 25 °C (lit.)

cation traces

Fe: ≤1  ppm
Pb: ≤1  ppm
Zn: ≤1  ppm

storage temp.

15-25°C

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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General description

EMSURE grade solvents are suitable for a broad spectrum of classical lab applications, and are frequently used in regulated and highly demanding lab applications. EMSURE provides worldwide best and most extensive product specifications. We declare our EMSURE range to be in compliance with the ACS, with the reagent part of the European Pharmacopoeia (Reag. Ph Eur) and also with the ISO standards.

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Analysis Note

Assay (N; Kjeldahl): ≥ 99.2 %
Identity (IR): passes test
Appearance: Clear, colorless liquid.
Colour (APHA): ≤ 10 APHA
Density (d 20 °C/ 4 °C): 1.132 - 1.135
Solidification point: 2.0 - 3.0 °C
Boiling point (2 kPa): about 103
Chloride (Cl): ≤ 1 ppm
Cd (Cadmium): ≤ 1 ppm
Cu (Copper): ≤ 1 ppm
Fe (Iron): ≤ 1 ppm
Pb (Lead): ≤ 1 ppm
Zn (Zinc): ≤ 1 ppm
Formic acid: ≤ 0.02 %
Ammonium Formate: ≤ 0.1 %
Sulfated ash (600 °C): ≤ 0.005 %
Water (according to Karl Fischer): ≤ 0.1 %

Legal Information

EMSURE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Target Organs

Blood

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C

Certificate of Analysis

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Certificate of Quality

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