203350

Blasticidin S, Hydrochloride, Streptomyces griseochromogenes

Blasticidin S, CAS 2079-00-7, a nucleoside antibiotic inhibits protein synthesis in prokaryotes and eukaryotes.

• Synonym(s): Blasticidin S, Hydrochloride, Streptomyces griseochromogenes
• Empirical Formula (Hill Notation): C₁₇H₂₆N₈O₅ · xHCl
• CAS Number: 3513-03-9
• Molecular Weight: 422.44 (free base basis)
• MDL number: MFCD02091640
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: white to off-white
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Nucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for selection of cells carrying plasmids conferring blasticidin resistance. Blasticidin resistance is conferred by the blasticidin S deaminase genes (bsr from Bacillus cereus or BSD from Aspergillus terreus), whose products convert blasticidin S to a non-toxic deaminohydroxy derivative.

Nucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for use as a dominant selectable marker in conjunction with blasticidin S resistant plasmids. Blasticidin S resistance is conferred by the blasticidin S deaminase gene (bcr), which converts blastisidin S to a nontoxic deaminohydroxy derivative.

Biochem/physiol Actions

Primary Target
protein synthesis

Warning

Toxicity: Highly Toxic (H)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Aqueous stock solutions (pH ≤7) are stable for up to 1 year at -20°C. Avoid alkaline pH. Do not subject to freeze/thaw cycles; upon thawing any aliquots, discard the unused portion.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Kimura, M., et al. 1994. Biochim. Biophys. Acta1219, 653.
Kimura, M., et al. 1994, Mol. Gen. Genet.242, 121.
Kojima, N., et al. 1994. J. Biol. Chem.269, 30451.
Kudo, T., et al. 1994. J. Immunol. Methods177, 17.
Sutoh, K. 1993. Plasmid30, 150.
Izumi, M., et al. 1991. Exp. Cell Res.197, 229.
Yamaguchi, H., et al. 1965. J. Biol. Chem.57, 667.
Takeuchi, S., et al. 1958. J. Antibiot.11, 1.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Statements: H300
• Precautionary Statements: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 1 Oral
• Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable