203351-M

Blasticidin S, Hydrochloride, Streptomyces sp., Sterile-Filtered Aqueous Solution, Cell Culture-Tested - CAS 589205 - Calbiochem

sterile; sterile-filtered, suitable for cell culture

• Synonym(s): Blasticidin S, Hydrochloride, Streptomyces sp., Sterile-Filtered Aqueous Solution, Cell Culture-Tested - CAS 589205 - Calbiochem
• Empirical Formula (Hill Notation): C₁₇H₂₆N₈O₅ · xHCl
• CAS Number: 3513-03-9
• Molecular Weight: 422.44 (free base basis)
• MDL number: MFCD02091640

Properties

• sterility: sterile; sterile-filtered
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• storage temp.: −20°C

Description

General description

Nucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for selection of cells carrying plasmids conferring blasticidin resistance. Blasticidin resistance is conferred by the blasticidin S deaminase gene (bsr) that converts blastisidin S to a nontoxic deaminohydroxy derivative.

Warning

Toxicity: Highly Toxic (H)

Physical form

Supplied at 10 mg/ml in 20 mM HEPES, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Kimura, M., et al. 1994. Biochim. Biophys. Acta1219, 653.
Kimura, M., et al. 1994. Mol. Gen. Genet.242, 121.
Kojima, N., et al. 1994. J. Biol. Chem.269, 30451.
Kudo, T., et al. 1994. J. Immunol. Methods177, 17.
Sutoh, K. 1993. Plasmid30, 150.
Izumi, M., et al. 1991. Exp. Cell Res.197, 229.
Yamaguchi, H., et al. 1965. J. Biol. Chem.57, 667.
Takeuchi, S., et al. 1958. J. Antibiot.11, 1.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Statements: H301
• Precautionary Statements: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Oral
• Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable