Merck
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69452-M

Sigma-Aldrich

HMS174 Competent Cells - Novagen

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

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HMS174(DE3) Competent Cells - Novagen HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

Sigma-Aldrich

69453-M

HMS174(DE3) Competent Cells - Novagen

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

HMS174(DE3)pLysS Competent Cells - Novagen HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. T7 lysozyme expression suppresses basal T7 expression.

Sigma-Aldrich

69454

HMS174(DE3)pLysS Competent Cells - Novagen

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. T7 lysozyme expression suppresses basal T7 expression.

BL21(DE3) Singles Competent Cells - Novagen BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

Sigma-Aldrich

70235-M

BL21(DE3) Singles Competent Cells - Novagen

BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

BLR(DE3) Competent Cells - Novagen BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.

Sigma-Aldrich

69053

BLR(DE3) Competent Cells - Novagen

BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.

BL21(DE3) Competent Cells - Novagen BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

Sigma-Aldrich

69450-M

BL21(DE3) Competent Cells - Novagen

BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

BL21(DE3) Chemically Competent Cells for protein expression

Sigma-Aldrich

CMC0014

BL21(DE3) Chemically Competent Cells

for protein expression

NovaBlue Competent Cells - Novagen NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.

Sigma-Aldrich

69825-M

NovaBlue Competent Cells - Novagen

NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.

Rosetta(DE3) Competent Cells - Novagen Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

70954

Rosetta(DE3) Competent Cells - Novagen

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Rosetta-gami B Competent Cells - Novagen Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.

Sigma-Aldrich

71135

Rosetta-gami B Competent Cells - Novagen

Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.

OverExpress™ C41(DE3) Chemically Competent Cells for the highest protein expression

Sigma-Aldrich

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells

for the highest protein expression

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

cell transformation

transformation efficiency: >5×106 cfu/μg

storage temp.

−70°C

Related Categories

General description

Genotype: F-recA1 hsdR(rK12- mK12+) (Rif R)





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HMS174 strains provide the recA mutation in a K-12 background. Like BLR, these strains may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

Packaging

1 ml in Glass bottle
0.4 ml in Plastic ampoule

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlHMS174 Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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