Merck
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69956

Sigma-Aldrich

BLR(DE3)pLysS Competent Cells - Novagen

BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences. T7 lysozyme expression suppresses basal T7 expression.

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BLR(DE3) Competent Cells - Novagen BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.

Sigma-Aldrich

69053

BLR(DE3) Competent Cells - Novagen

BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.

HMS174(DE3)pLysS Competent Cells - Novagen HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. T7 lysozyme expression suppresses basal T7 expression.

Sigma-Aldrich

69454

HMS174(DE3)pLysS Competent Cells - Novagen

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. T7 lysozyme expression suppresses basal T7 expression.

Rosetta-gami B Competent Cells - Novagen Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.

Sigma-Aldrich

71135

Rosetta-gami B Competent Cells - Novagen

Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.

pET-32 Ek/LIC Vector Kit - Novagen Novagen′s pET-32 Ek/LIC vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx-Tag thioredoxin protein and His-Tag and S-Tag thioredoxin protein

Sigma-Aldrich

69076

pET-32 Ek/LIC Vector Kit - Novagen

Novagen′s pET-32 Ek/LIC vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx-Tag thioredoxin protein and His-Tag and S-Tag thioredoxin protein

Rosetta(DE3) Competent Cells - Novagen Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

70954

Rosetta(DE3) Competent Cells - Novagen

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Rosetta 2(DE3) Competent Cells - Novagen Novagen′s Rosetta 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

71397

Rosetta 2(DE3) Competent Cells - Novagen

Novagen′s Rosetta 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

BL21(DE3) Competent Cells - Novagen BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

Sigma-Aldrich

69450-M

BL21(DE3) Competent Cells - Novagen

BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

BL21(DE3)pLysS Singles Competent Cells - Novagen

Sigma-Aldrich

70236-M

BL21(DE3)pLysS Singles Competent Cells - Novagen

Tuner Competent Cells - Novagen Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

Sigma-Aldrich

70622-M

Tuner Competent Cells - Novagen

Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

BL21(DE3) Chemically Competent Cells for protein expression

Sigma-Aldrich

CMC0014

BL21(DE3) Chemically Competent Cells

for protein expression

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

cell transformation

transformation efficiency: >2×106 cfu/μg

storage temp.

−70°C

Related Categories

General description

Genotype: F-ompT hsdSB(rB- mB-) gal dcm (DE3) Δ(srl-recA)306::Tn10 pLysS (CamR, TetR)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
BLR is a recA- derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences or whose products may cause the loss of the DE3 prophage (1, 2).

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences. T7 lysozyme expression suppresses basal T7 expression.

Packaging

1 ml in Glass bottle
0.4 ml in Plastic ampoule

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlBLR(DE3)pLysS Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Other Notes

1. A. Roca (U. of Wisconsin), personal
communication.

2. Studier, F. W. (1991) J. Mol. Biol. 219,
37-44.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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ISHL Notified Names

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Certificate of Analysis

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Certificate of Quality

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