Merck
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70236-M

Sigma-Aldrich

BL21(DE3)pLysS Singles Competent Cells - Novagen

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BL21(DE3)pLysS Competent Cells - Novagen BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.

Sigma-Aldrich

69451-M

BL21(DE3)pLysS Competent Cells - Novagen

BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.

BL21(DE3) Chemically Competent Cells for protein expression

Sigma-Aldrich

CMC0014

BL21(DE3) Chemically Competent Cells

for protein expression

BL21(DE3) Competent Cells - Novagen BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

Sigma-Aldrich

69450-M

BL21(DE3) Competent Cells - Novagen

BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

Rosetta 2(DE3) Competent Cells - Novagen Novagen′s Rosetta 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

71397

Rosetta 2(DE3) Competent Cells - Novagen

Novagen′s Rosetta 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Rosetta(DE3) Competent Cells - Novagen Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

70954

Rosetta(DE3) Competent Cells - Novagen

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

BL21(DE3) Electrocompetent Cells for protein expression

Sigma-Aldrich

CMC0016

BL21(DE3) Electrocompetent Cells

for protein expression

OverExpress™ C41(DE3) Chemically Competent Cells for the highest protein expression

Sigma-Aldrich

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells

for the highest protein expression

Rosetta 2(DE3)pLysS Singles Competent Cells - Novagen Novagen′s Rosetta 2 (pLysS) host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli along with T7 lysozyme activity.

Sigma-Aldrich

71401-M

Rosetta 2(DE3)pLysS Singles Competent Cells - Novagen

Novagen′s Rosetta 2 (pLysS) host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli along with T7 lysozyme activity.

Rosetta 2 Competent Cells - Novagen Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

71402-M

Rosetta 2 Competent Cells - Novagen

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

SIG10 Chemically Competent Cells for protein expression and DNA plasmid production

Sigma-Aldrich

CMC0001

SIG10 Chemically Competent Cells

for protein expression and DNA plasmid production

manufacturer/tradename

Novagen®

Quality Level

100

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

cell transformation

transformation efficiency: >2×107 cfu/μg

storage temp.

−70°C

Related Categories

General description

Genotype: F-ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

Packaging

11, 22 reactions in Glass bottle

Components

•11 or 22Singles Competent Cells•2 or 4 × 2 mlSOC Medium•10 µlTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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Certificate of Analysis

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Certificate of Quality

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