Merck
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71227

Sigma-Aldrich

NovaBlue GigaSingles Competent Cells - Novagen

NovaBlue GigaSingles Competent Cells produce greater than 1 × 10⁹ colonies/µg plasmid DNA for cloning applications requiring high-efficiency transformations.

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NovaBlue Singles Competent Cells - Novagen NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.

Sigma-Aldrich

70181-M

NovaBlue Singles Competent Cells - Novagen

NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.

BLR(DE3) Competent Cells - Novagen BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.

Sigma-Aldrich

69053

BLR(DE3) Competent Cells - Novagen

BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.

BL21(DE3) Competent Cells - Novagen BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

Sigma-Aldrich

69450-M

BL21(DE3) Competent Cells - Novagen

BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

BL21(DE3) Chemically Competent Cells for protein expression

Sigma-Aldrich

CMC0014

BL21(DE3) Chemically Competent Cells

for protein expression

BL21(DE3) Singles Competent Cells - Novagen BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

Sigma-Aldrich

70235-M

BL21(DE3) Singles Competent Cells - Novagen

BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

pET-51b(+) DNA - Novagen Novagen′s pET-51b(+) vector is designed for cloning and high-level expression of target proteins fused with the 8 aa Strep-Tag II coding sequence that is cleavable with enterokinase (Ek) protease.

Sigma-Aldrich

71553-M

pET-51b(+) DNA - Novagen

Novagen′s pET-51b(+) vector is designed for cloning and high-level expression of target proteins fused with the 8 aa Strep-Tag II coding sequence that is cleavable with enterokinase (Ek) protease.

HMS174(DE3) Competent Cells - Novagen HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

Sigma-Aldrich

69453-M

HMS174(DE3) Competent Cells - Novagen

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

SIG10 Chemically Competent Cells for protein expression and DNA plasmid production

Sigma-Aldrich

CMC0001

SIG10 Chemically Competent Cells

for protein expression and DNA plasmid production

Ion-Ex Polishing Cartridge L 55 cm (22 in.), for use with Super-Q®

Milli-Q

CPMB02202

Ion-Ex Polishing Cartridge

L 55 cm (22 in.), for use with Super-Q®

NovaBlue Competent Cells - Novagen NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.

Sigma-Aldrich

69825-M

NovaBlue Competent Cells - Novagen

NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze

cell transformation

transformation efficiency: 1×109 cfu/μg

storage temp.

−70°C

Related Categories

General description

NovaBlue GigaSingles Competent Cells produce greater than 1 × 10⁹ colonies/µg plasmid DNA for cloning applications requiring high-efficiency transformations.
NovaBlue competent cells are for routine molecular cloning applications. NovaBlue is a K-12 strain derivative that offers high transformation efficiency,facilitates plasmid stability, and allows blue/whitescreening with appropriate plasmids.
NovaBlue GigaSingles Competent Cellsproduce greater than 1 × 109 colonies/µgplasmid DNA for cloning applicationsrequiring high-efficiency transformations.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Packaging

1, 4 pkg
11, 22 reactions

Features and Benefits

  • Guaranteed efficiency >1 × 10⁹ cfu/µg
  • Ideal for high-efficiency cloning
  • High-quality plasmid DNA preparation
  • Easy-to-use Singles format
  • Prepared using an optimized chemical method

Components

•11 or 22 × 50 µlCompetent Cells

•2 or 4 × 2 mlSOC Medium

•10 µlTest Plasmid (ampicillin resistant)

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

GigaSingles is a trademark of EMD Biosciences, Inc., Madison, WI USA
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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