Merck
  • 71341
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71341

Sigma-Aldrich

pRSFDuet-1 DNA - Novagen

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pACYCDuet-1 DNA - Novagen pACYCDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is receded by a T7 promoter, lac operator and ribosome binding site.

Sigma-Aldrich

71147

pACYCDuet-1 DNA - Novagen

pACYCDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is receded by a T7 promoter, lac operator and ribosome binding site.

pCDFDuet-1 DNA - Novagen

Sigma-Aldrich

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pCDFDuet-1 DNA - Novagen

pETDuet-1 DNA - Novagen pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites, each of which is preceded by a T7 promoter, lac operator and ribosome binding sites.

Sigma-Aldrich

71146

pETDuet-1 DNA - Novagen

pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites, each of which is preceded by a T7 promoter, lac operator and ribosome binding sites.

pCOLADuet-1 DNA - Novagen®

Sigma-Aldrich

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pCOLADuet-1 DNA - Novagen®

pCDF-1b DNA - Novagen

Sigma-Aldrich

71330-M

pCDF-1b DNA - Novagen

pET-51b(+) DNA - Novagen Novagen′s pET-51b(+) vector is designed for cloning and high-level expression of target proteins fused with the 8 aa Strep-Tag II coding sequence that is cleavable with enterokinase (Ek) protease.

Sigma-Aldrich

71553-M

pET-51b(+) DNA - Novagen

Novagen′s pET-51b(+) vector is designed for cloning and high-level expression of target proteins fused with the 8 aa Strep-Tag II coding sequence that is cleavable with enterokinase (Ek) protease.

pCDF-2 Ek/LIC Vector Kit - Novagen

Sigma-Aldrich

71337-M

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BL21(DE3) Chemically Competent Cells for protein expression

Sigma-Aldrich

CMC0014

BL21(DE3) Chemically Competent Cells

for protein expression

pT7-FLAG™-2 Expression Vector

Sigma-Aldrich

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pT7-FLAG™-2 Expression Vector

Rosetta 2(DE3) Singles Competent Cells - Novagen Novagen′s Rosetta 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

71400-M

Rosetta 2(DE3) Singles Competent Cells - Novagen

Novagen′s Rosetta 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

form

liquid

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

−70°C

General description

pRSFDuet-1 is designed for the co-expression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (RBS). The vector also carries the pRSF1030 replicon (also known as NTP1), LacI gene, and kanamycin resistance marker.

Application

pRSFDuet-1 DNA - Novagen has been used as a vector:
  • to clone the DNA fragments of defensin α 5 (DEFA5) for recombinant expression
  • to clone the coding region of Mad1597–719
  • to clone the globular domain of Bombyx mori Spc24 (73-162aa) with an N-terminal 6X His-tag to generate the B. mori 6xHis-Spc24(73-162)-Spc25(70-211) pRSF-DUET1 bacterial expression constructs

Packaging

10 μg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

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