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71402-M

Sigma-Aldrich

Rosetta 2 Competent Cells - Novagen

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Quality Level

manufacturer/tradename

Novagen®

storage condition

OK to freeze

cell transformation

transformation efficiency: >2 × 106 cfu/μg

storage temp.

−70°C

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709537105869825-M
manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

cell transformation

transformation efficiency: >2 × 106 cfu/μg

cell transformation

transformation efficiency: >2×106 cfu/μg

cell transformation

transformation efficiency: >1×106 cfu/μg

cell transformation

transformation efficiency: >1×108 cfu/μg

General description

Genotype: F-ompT hsdSB(rB- mB-) gal dcm pRARE2 (CamR)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta 2 Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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