Merck
  • 71553M
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71553-M

Sigma-Aldrich

pET-51b(+) DNA - Novagen

Novagen′s pET-51b(+) vector is designed for cloning and high-level expression of target proteins fused with the 8 aa Strep-Tag II coding sequence that is cleavable with enterokinase (Ek) protease.

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pETDuet-1 DNA - Novagen pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites, each of which is preceded by a T7 promoter, lac operator and ribosome binding sites.

Sigma-Aldrich

71146

pETDuet-1 DNA - Novagen

pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites, each of which is preceded by a T7 promoter, lac operator and ribosome binding sites.

pET-49b(+) DNA - Novagen Novagen′s pET-49b(+) vector is designed for cloning and high-level expression of target proteins fused with the 220 aa schistosomal glutathione-S-transferase-Tag protein.

Sigma-Aldrich

71463-M

pET-49b(+) DNA - Novagen

Novagen′s pET-49b(+) vector is designed for cloning and high-level expression of target proteins fused with the 220 aa schistosomal glutathione-S-transferase-Tag protein.

Sigma-Aldrich

Sigma-Aldrich

70608

pETBlue-1 DNA - Novagen

Novagen′s pETBlue-1 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes.

Rosetta(DE3) Competent Cells - Novagen Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

70954

Rosetta(DE3) Competent Cells - Novagen

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

pET-32 Ek/LIC Vector Kit - Novagen Novagen′s pET-32 Ek/LIC vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx-Tag thioredoxin protein and His-Tag and S-Tag thioredoxin protein

Sigma-Aldrich

69076

pET-32 Ek/LIC Vector Kit - Novagen

Novagen′s pET-32 Ek/LIC vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx-Tag thioredoxin protein and His-Tag and S-Tag thioredoxin protein

BL21(DE3) Chemically Competent Cells for protein expression

Sigma-Aldrich

CMC0014

BL21(DE3) Chemically Competent Cells

for protein expression

pET-32 Xa/LIC Vector Kit - Novagen Novagen′s pET-32 Xa/LIC vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx-Tag thioredoxin protein, His-Tag and S-Tag coding sequences.

Sigma-Aldrich

70072-M

pET-32 Xa/LIC Vector Kit - Novagen

Novagen′s pET-32 Xa/LIC vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx-Tag thioredoxin protein, His-Tag and S-Tag coding sequences.

BL21(DE3) Competent Cells - Novagen BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

Sigma-Aldrich

69450-M

BL21(DE3) Competent Cells - Novagen

BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

pT7-FLAG™-2 Expression Vector

Sigma-Aldrich

P1243

pT7-FLAG™-2 Expression Vector

BL21 Competent Cells - Novagen BL21 host strain is the most widely used host background and has the advantage of being deficient in both lon and ompT proteases.

Sigma-Aldrich

69449

BL21 Competent Cells - Novagen

BL21 host strain is the most widely used host background and has the advantage of being deficient in both lon and ompT proteases.

Quality Level

100

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

storage temp.

−70°C

General description

The pET-51b(+) vector is designed for cloning and high-level expression of target proteins fused with the 8 aa Strep•Tag II coding sequence that is cleavable with enterokinase (Ek) protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Ek protease cleavage site (AspAspAspAspLys), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence with 10 histidines is compatible with purification, detection, and quantification.

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

Packaging

10 μg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificate of Quality

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