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SCR020

Sigma-Aldrich

Mesenchymal Adipogenesis Kit

Mesenchymal Stem Cell Adipogenesis Kit typically obtains 30% mature adipocytes from the rat bone marrow derived mesenchymal stem cells.

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Synonym(s):
Adipocyte Differentiation Media
eCl@ss:
32161000

Quality Level

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type mesenchymal stem cell(s)

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Chemicon®

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Chemicon®

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input

sample type mesenchymal stem cell(s)

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sample type mesenchymal stem cell(s)

input

sample type mesenchymal stem cell(s)

input

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Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

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General description

Introduction:

Excessive weight gain and obesity pose significant health challenges to many industrialized nations. Many metabolic disorders associated with cardiovascular diseases such as diabetes and atherosclerosis are directly linked to the increased production and size of adipose cells. Understanding the molecular mechanism that underlie adipogenesis, the process by which adipose or fat tissue is formed, is thus of critical importance. Elucidation of the steps involved in adipogenesis, including terminal differentiation, have been facilitated in large part by the development of pre-adipocyte cell lines such as the Swiss 3T3-L1 mouse model (Please refer to CHEMICON′s Adipogenesis Assay Kit, Catalog No. ECM950). However using these model systems to study some of the earliest events in cellular differentiation are problematic as these cells are already lineage-restricted and pre-determined to become adipocytes.

Stem cell technology, particularly embryonic stem cells and/or mesenchymal stem cells offer attractive alternative sources of adipocytes for tissue culture studies and for the biochemical dissection of the earliest steps involved in adipose cell determination. Mesenchymal stem cells are multipotent progenitor cells that have the capacity to differentiate into several mesenchymal cell lineages, including bone, cartilage and fat.
Millipore′s Mesenchymal Stem Cell Adipogenesis Kit contains reagents that readily differentiate mesenchymal stem cells to an adipogenic lineage as assessed with Oil Red O staining of lipid vacuoles in mature adipocytes. These factors include dexamethasone, IBMX, insulin and indomethacin. Along with Oil Red O staining solution, a hematoxylin solution is provided to counterstain the cell nucleus.

Using Millipore′s Mesenchymal Stem Cell Adipogenesis Kit, we typically obtain >30% mature adipocytes from the rat bone marrow derived mesenchymal stem cells. Efficiency of adipogenic differentiation may vary, depending upon the quality of the mesenchymal stem cells and if variations to the protocol are introduced.

For Research Use Only; not for use in diagnostic procedure.

Application

Research Category
Stem Cell Research

Components

Dexamethasone Solution: (Catalog No. 90357) One vial containing 100 μL of 10 mM Dexamethasone in ethanol. Store at -20 °C.

IBMX Solution: (Catalog No. 90355) One vial containing 250 μL of 0.5 M 3-isobutyl-1-methylxanthine (IBMX) in DMSO. Store at -20°C.

Insulin Solution: (Catalog No. 90356) Two vials containing 250 μL of 10 mg/mL recombinant human insulin. Store at -20°C.

Indomethacin Solution: (Catalog No. 2003626) Three vials containing 1 mL of 10 mM Indomethacin in methanol. Store at -20°C.

Oil Red O Solution: (Catalog No. 90358) One bottle containing 60 mL 0.36% Oil Red O Solution in 60% isopropanol. Store at room temperature.

Hematoxylin Solution: (Catalog No. 2003732) One bottle containing 50 mL Hematoxylin. Store at room temperature.

Storage and Stability

Note: Kit components require two different storage temperatures.

Dexamethasone Solution, IBMX Solution, Insulin Solution and Indomethacin Solution should be stored at -20°C. Oil Red O and Hematoxylin Solutions should be stored at room temperature. Storage of Oil Red O Solution at -20°C may result in formation of insoluble precipitates and is not recommended. If Oil Red O solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

Precautions:
Oil Red O stains skin and clothing. IBMX, Dexamethasone and Indomethacin are irritants and potentially toxic. DMSO is readily absorbed through the skin. Wear a lab coat and gloves when handling these solutions.

Isopropanol is flammable. Keep solutions containing isopropanol (Oil Red O Solution) away from open flames.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Repr. 1B - STOT RE 2 Oral - STOT SE 1 - STOT SE 2 - STOT SE 3

Target Organs

Central nervous system, Eyes, Kidney

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

48.2 °F

Flash Point(C)

9 °C


Regulatory Listings

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PDSCL

Please refer to KIT Component information

PRTR

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FSL

Please refer to KIT Component information

ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

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JAN Code

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Oxidative medicine and cellular longevity, 2013, 234179-234179 (2013-04-17)
After injury to the CNS, microglia are rapidly activated and concentrated and trigger inflammatory reaction at the sites of injury. Bone marrow mesenchymal stem cells (BMMSC) represent attractive cell sources for treating CNS injury. Although anti-inflammatory and paracrine effects of
Genetically-modified pig mesenchymal stromal cells: xenoantigenicity and effect on human T-cell xenoresponses.
Mohamed Ezzelarab,Corin Ezzelarab,Tyler Wilhite,Goutham Kumar,Hidetaka Hara,David Ayares et al.
Xenotransplantation null
S B Bahrami et al.
Integrative biology : quantitative biosciences from nano to macro, 9(3), 223-237 (2017-02-22)
Hyaluronan, CD44 and the Receptor for Hyaluronan-Mediated Motility (RHAMM, gene name HMMR) regulate stem cell differentiation including mesenchymal progenitor differentiation. Here, we show that CD44 expression is required for subcutaneous adipogenesis, whereas RHAMM expression suppresses this process. We designed RHAMM
Nagasuryaprasad Kotikalapudi et al.
Scientific reports, 11(1), 16983-16983 (2021-08-22)
Obesity (Ob) poses a significant risk factor for the onset of metabolic syndrome with associated complications, wherein the Mesenchymal Stem Cell (MSC) therapy shows pre-clinical success. Here, we explore the therapeutic applications of human Placental MSCs (P-MSCs) to address Ob-associated
Differential properties of human ACL and MCL stem cells may be responsible for their differential healing capacity.
Zhang, J; Pan, T; Im, HJ; Fu, FH; Wang, JH
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