- SCR103
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SCR103
Collagenase type I
The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.
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Synonym(s):
Collagenase Type I from Clostridium histolyticum, Tissue dissociation
eCl@ss:
32160801
NACRES:
NA.75
Recommended Products
Quality Level
form
powder
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
input
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
shipped in
wet ice
Related Categories
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This Item | COLLA-RO | COLLD-RO | COLLB-RO |
|---|---|---|---|
| form liquid | form lyophilized | form lyophilized | form lyophilized |
| manufacturer/tradename Chemicon® | manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche |
| technique(s) cell culture | stem cell: suitable | technique(s) - | technique(s) - | technique(s) - |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| input sample type induced pluripotent stem cell(s) | input - | input - | input - |
General description
Collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means. The preparation also may contain caseinase, clostripain, and tryptic activities. This preparation can be used to dissociate cells in culture, and has been successfully used to passage human embryonic stem cells cultured with Millipore’s HEScGROTM Animal Component-Free Medium.
Packaging
1 vial containing 250mg of lyophilized powder
Quality
Each lot of collagenase type I is assayed via a modification of Mandl (1964), with the extent of collagen breakdown determined using the colorimetric ninhydrin method of Moore and Stein (1948). Each lot is then tested for successful passage of human embryonic stem cells cultured in Millipore′s HEScGRO Medium for hES cells. Cells are characterized for correct marker expression and morphology after five passages.
Physical form
Collagenase type I is supplied as a dialyzed, lyophilized powder.
Storage and Stability
Collagenase type I should be stored in its powdered form at 2-8oC until ready to use. Solutions of collagenase type I can be aliquotted and stored at -20oC, but should not be repeatedly thawed and re-frozen.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificate of Analysis
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Certificate of Quality
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