Merck
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RDLIG-RO

Roche

Rapid DNA Dephos & Ligation Kit

sufficient for 40 reactions (04898117001), sufficient for 160 reactions (04898125001), optimum reaction temp. 37 °C

Synonym(s):
cloning of dna fragments, dna ligase, ligases
Enzyme Commission number:

usage

sufficient for 160 reactions (04898125001)
sufficient for 40 reactions (04898117001)

Quality Level

manufacturer/tradename

Roche

parameter

37 °C optimum reaction temp.

storage temp.

−20°C

General description

The Rapid DNA Dephos & Ligation Kit enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments.

Specificity

Specificity: rAPid Alkaline Phosphatase
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.

Application

The Rapid DNA Dephos & Ligation Kit minimizes the time needed to perform diverse applications, including:
  • Cloning of fragments into either plasmid or phage vectors
  • Linker ligation
  • Recircularization of linear vector DNA
  • Generation of libraries

Packaging

1 kit containing 5 components

Unit Definition

Unit Definition: rAPid Alkaline Phosphatase
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one μmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.

Volume Activity: rApid Alkaline Phosphatase: 1 U/μl
T4 DNA Ligase: 5 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • rAPid Alkaline Phosphatase Buffer 10x concentrated

  • rAPid Alkaline Phosphatase 1 U/μl

  • DNA Dilution Buffer 5x concentrated

  • T4 DNA Ligation Buffer 2x concentrated

  • T4 DNA Ligase 5 U/μl

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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