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69385

Supelco

Protein Standard Mix 15 - 600 kDa

for size exclusion chromatography

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NACRES:
NA.24

Quality Level

form

solid

mol wt

15-600 kDa

analyte chemical class(es)

amino acids, peptides, proteins

technique(s)

gel permeation chromatography (GPC): suitable

application(s)

clinical
food and beverages
pharmaceutical

format

neat

storage temp.

−20°C

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P8119P5494P5369
Supelco

69385

Protein Standard Mix 15 - 600 kDa

Protein Standard analytical standard, 80 mg/mL (HSA and gamma-globulins)

P8119

Protein Standard

Supelco

P5494

Protein Standard Set

Supelco

P5369

Protein Standard

format

neat

format

multi-component solution

format

neat

format

single component solution

mol wt

15-600 kDa

mol wt

-

mol wt

-

mol wt

-

technique(s)

gel permeation chromatography (GPC): suitable

technique(s)

gel permeation chromatography (GPC): suitable

technique(s)

gel permeation chromatography (GPC): suitable

technique(s)

gel permeation chromatography (GPC): suitable

analyte chemical class(es)

amino acids, peptides, proteins

analyte chemical class(es)

amino acids, peptides, proteins

analyte chemical class(es)

amino acids, peptides, proteins

analyte chemical class(es)

amino acids, peptides, proteins

application(s)

clinical
food and beverages
pharmaceutical

application(s)

food and beverages

application(s)

food and beverages

application(s)

food and beverages

General description

The protein standard mix is a calibration standard to test and monitor performance of size exclusion chromatography (SEC) columns. It is a lyophilized mixture of molecular weight markers ranging from 15 kDa to 600 kDa.

Components

Thyroglobulin bovine MW ~ 670 000 Da

γ-globulins from bovine blood MW ~ 150 000 Da

Ovalbumin MW~ 44 300 Da

Ribonuclease A type I-A MW ~ 13 700 Da

p-aminobenzoic acid (pABA) MW ~ 137 Da

Application

This analytical standard is used for the following:
  • Evaluation of selectivity and separation efficiency of size exclusion chromatography (SEC) to separate intact proteins by varying flow rate, size of silica particles and pore sizes in the column
  • Simultaneous determination of oligomerized and nitrated proteins by size exclusion chromatography-high performance liquid chromatography-diode array detection (SEC-HPLC-DAD)
  • Molecular weight separation of proteins by size-exclusion chromatography, formed upon O3 and NO2 induced oxidation, nitration, and oligomerization of bovine serum albumin (BSA) as a model protein
  • Estimation of molecular masses of two recombinant proteins— TNF fluorescent sensor (BTN-Kat) and fluorescent sensor-inhibitor (ITN-Kat), by size exclusion chromatography (SEC) to evaluate their ability of binding and neutralizing tumor necrosis factor (TNF) in vitro and further serving as imaging labels for non-invasive analysis

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Poisonous substance

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

69385-30MG:4.548173339788E12


Certificates of Analysis (COA)

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T1503
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25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Articles

Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.

A complete SEC-UV workflow for the characterization of mAb monomers, aggregates, and fragments using Zenix® and Zenix®-C SEC columns, including system suitability testing and forced pH and temperature stress studies.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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