D9564

DAPI, dilactate

≥98% (HPLC)

• Synonym(s): 4′,6-Diamidino-2-phenyindole, dilactate
• Empirical Formula (Hill Notation): C₁₆H₁₅N₅ · 2C₃H₆O₃
• Molecular Weight: 457.48
• PubChem Substance ID: 329798905
• NACRES: NA.47

Properties

• Quality Level: 300
• assay: ≥98% (HPLC)
• form: powder
• solubility: H₂O: 20 mg/mL
• ε (extinction coefficient): 28.00-32.50 at 345-351 nm in methanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

Description

General description

DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.

Application

DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.

Packaging

10 mg

Biochem/physiol Actions

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves