F9252
Folin & Ciocalteu′s phenol reagent
suitable for determination of total protein by Lowry method, 1.9-2.1 N
About This Item
Recommended Products
form
liquid
Quality Level
concentration
1.9-2.1 N
color
clear yellow
faint yellow to very dark yellow, and Faint Green-Yellow to Very Dark Green-Yellow
pH
<0.5 (20 °C)
density
1.240 g/cm3 at 20 °C
suitability
suitable for determination of total protein by Lowry method
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
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General description
stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750nm) or shoulder (660nm) are used to quantitate protein concentrations between 1-100 mg/ml while absorbance at 550nm is used to quantitate higher protein concentrations.
In the absence of copper, color intensity is determined primarily by the tyrosine and tryptophan content of the protein, and to a lesser extent by cysteine and histidine. Copper(II) has no effect on color formation by tyrosine, tryptophan, or histidine, but reduces color formation due to cysteine.
Many modifications of the original assay procedure have been published, including methods for enhancing the color development, for determining the content of insoluble proteins, and for automating the procedure. Compounds including many buffers, chelating agents, detergents, and cyclic organic compounds can interfere with the Lowry protein assay.
Folin & Ciocalteu′s phenol reagent can also be used as a spray reagent in chromatographic procedures.
Application
Other Notes
Linkage
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
ISHL Indicated Name
Substances Subject to be Indicated Names
ISHL Notified Names
Substances Subject to be Notified Names
JAN Code
F9252-10L:
F9252-1EA:
F9252-5L:
F9252-BULK:
F9252-100ML:4548173949178
F9252-500ML:4548173949192
F9252-10ML-KC:
F9252-20L:
F9252-1EA-KC:
F9252-10ML:
F9252-1L:4548173949185
F9252-VAR:
F9252-50L:
F9252-PROC:
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Protocols
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Related Content
Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
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