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G9012

Sigma-Aldrich

Glycerol

≥99.5%

Synonym(s):
1,2,3-Propanetriol, Glycerin
Linear Formula:
HOCH2CH(OH)CH2OH
CAS Number:
Molecular Weight:
92.09
Beilstein:
635685
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

vapor density

3.1 (vs air)

Quality Level

vapor pressure

<1 mmHg ( 20 °C)

Assay

≥99.5%

form

viscous liquid

autoignition temp.

698 °F

impurities

≤0.20% Water (Karl Fischer)

refractive index

n20/D 1.474 (lit.)

bp

182 °C/20 mmHg (lit.)

mp

20 °C (lit.)

density

1.25 g/mL (lit.)

cation traces

heavy metals (as Pb): ≤5 ppm

λ

1 cm path

UV absorption

λ: 205 nm Amax: 1.0
λ: 225 nm Amax: 0.40
λ: 280 nm Amax: 0.07
λ: 320 nm Amax: 0.02
λ: 340 nm Amax: 0.01
λ: 400 nm Amax: 0.01

suitability

suitable for component for culture media

SMILES string

OCC(O)CO

InChI

1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2

InChI key

PEDCQBHIVMGVHV-UHFFFAOYSA-N

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This Item
G6279G551649767
Glycerol &#8805;99.5%

Sigma-Aldrich

G9012

Glycerol

Glycerol BioXtra, &#8805;99% (GC)

Sigma-Aldrich

G6279

Glycerol

Glycerol for molecular biology, &#8805;99.0%

Sigma-Aldrich

G5516

Glycerol

Glycerol BioUltra, for molecular biology, anhydrous, &#8805;99.5% (GC)

Sigma-Aldrich

49767

Glycerol

form

viscous liquid

form

liquid

form

liquid

form

viscous liquid

impurities

≤0.20% Water (Karl Fischer)

impurities

≤0.0005% Phosphorus (P)

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

mp

20 °C (lit.)

mp

20 °C (lit.)

mp

20 °C (lit.)

mp

20 °C (lit.)

cation traces

heavy metals (as Pb): ≤5 ppm

cation traces

Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH4+: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%

cation traces

Fe: ≤5 ppm, Mg: ≤5 ppm, heavy metals (as Pb): <5 ppm

cation traces

Ag: ≤5 mg/kg, Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, NH4+: ≤5 mg/kg, Na: ≤20 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Tl: ≤5 mg/kg, Zn: ≤1 mg/kg

suitability

suitable for component for culture media

suitability

-

suitability

-

suitability

-

General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Application

Glycerol has been used as a component of mounting medium for immunofluorescence. It has also been used as additives in poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) based inks.
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Biochem/physiol Actions

Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

390.2 °F - Pensky-Martens closed cup

Flash Point(C)

199 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

FSL

Flammable liquids
Type 3 petroleums
Hazardous rank III
Water soluble liquid


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Protocols

Immunoprecipitation Procedure

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

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