All Photos(1)

PHR1035

Supelco

Folic acid

Pharmaceutical Secondary Standard; Certified Reference Material

Synonym(s):
N-[4-[[(2-Amino-1,4-dihydro-4-oxo-6-pteridinyl)methyl]amino]benzoyl]-L-glutamic acid, PteGlu, Vitamin M, Pteroyl-L-glutamic acid
Empirical Formula (Hill Notation):
C19H19N7O6
CAS Number:
Molecular Weight:
441.40
Beilstein:
100781
EC Number:
MDL number:
eCl@ss:
34058014
PubChem Substance ID:
NACRES:
NA.24

Quality Level

grade

certified reference material
pharmaceutical secondary standard

form

neat

CofA

current certificate can be downloaded

application(s)

HPLC: suitable
gas chromatography (GC): suitable

Featured Industry

Pharmaceutical (small molecule)

format

neat

pharmacopeia traceability

traceable to PhEur F0300000
traceable to USP 1286005

SMILES string

NC(N1)=NC(C2=C1N=CC(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)=N2)=O

InChI

1S/C19H19N7O6/c20-19-25-15-14(17(30)26-19)23-11(8-22-15)7-21-10-3-1-9(2-4-10)16(29)24-12(18(31)32)5-6-13(27)28/h1-4,8,12,21H,5-7H2,(H,24,29)(H,27,28)(H,31,32)(H3,20,22,25,26,30)/t12-/m0/s1

InChI key

OVBPIULPVIDEAO-LBPRGKRZSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Folic acid is mostly obtained from the diet itself or from pharmaceutical drugs such as vitamin pills.† It is highly necessary to consume folic acid for the prevention of neural tube defects, mainly anencephaly and spina bifida.† It belongs to the class of water soluble B vitamins called folates. It is mainly used to fortify cereals/grains and dietary supplements.
Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

Application

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.
Folic acid may be used as a pharmaceutical reference standard for the quantification of the analyte in pharmaceutical formulations using ultraviolet spectrophotometric technique and microemulsion electrokinetic chromatography.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP (PhEur) and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

Footnote

To see an example of a Certificate of Analysis for this material enter LRAB7352 in the slot below. This is an example certificate only and may not be the lot that you receive.

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

Certificate of Analysis

Certificate of Origin

Quantitative determination of folic acid in multivitamin/multielement tablets using liquid chromatography/tandem mass spectrometry
Nelson.CB, et al.
Journal of Chromatography A, 1135, 203-211 (2006)
Determination of folic acid in tablets by microemulsion electrokinetic chromatography
Aurora-Prado SM, et al.
Journal of Chromatography A, 1051(1-2), 291-296 (2004)
A UV spectrophotometric method for the determination of folic acid in pharmaceutical tablets and dissolution tests
Matias R, et al.
Analytical Methods : Advancing Methods and Applications, 6(9), 3065-3071 (2014)
Séverine Ledoux et al.
Annals of surgery, 259(6), 1104-1110 (2014-05-14)
To study long-term nutritional deficits based on adherence to a standardized nutritional care after gastric bypass (GBP). Long-term prospective data on nutritional complications after GBP are missing. It is not known whether severe deficiencies are prevented by standard multivitamin supplementation...
Breige McNulty et al.
The American journal of clinical nutrition, 98(1), 92-98 (2013-05-31)
Supplementation with folic acid (FA) is recommended worldwide before and during early pregnancy because of its proven effect in preventing neural tube defects, but the role of FA after the 12th gestational week (GW) is much less clear. We investigated...

Articles

HPLC Analysis of Water-Soluble B-Vitamins on Ascentis® Express C18

Pyridoxine hydrochloride, ≥98% (HPLC); Nicotinamide, ≥98.5% (HPLC); Thiamine hydrochloride, reagent grade, ≥99% (HPLC); Biotin, ≥99.0% (T); Folic acid, analytical standard; Cyanocobalamin (B12), analytical standard; Riboflavin (B2), analytical standard

Protocols

HPLC Analysis of Vitamins, Water Soluble on Discovery® C18

Separation of Pyridoxine; Nicotinamide; Nicotinic acid; Folic acid; (−)-Riboflavin, meets USP testing specifications; L-Ascorbic acid

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service