Merck
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12103C

Sigma-Aldrich

Fetal Bovine Serum

USA origin, suitable for cell culture

Synonym(s):
Fetal Calf Sera, Fetal Calf Serum, FBS, FCS, sera, serum
MDL number:
NACRES:
NA.71

biological source

bovine fetus

Quality Level

product line

FBS Premier

composition

hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Application

Fetal Bovine Serum was used in different types of cell culture as a supplement, such as the following:
  • PC12 cells grown in Dulbecco′s modified Eagle′s medium supplemented with Fetal Bovine Serum along with other components.
  • HeLa cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium containing Fetal Bovine Serum along with other components.
  • BaF3 Cell Culture (cells were maintained in RPMI 1640 medium supplemented with Fetal Bovine Serum along with other components).
  • Primary hippocampal cell cultures (cells were maintained in Eagle′s minimum essential medium and supplemented with heat-inactivated fetal bovine serum along with other components).
  • HeLa, Cos1 and Cos7 cells were cultured in Dulbecco modified Eagle medium (DMEM) with fetal bovine serum along with other components.
Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.

This product is for further manufacturing use. THIS PRODUCT IS NOT INTENDED FOR HUMAN OR THERAPEUTIC USE.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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