54457
HEPES
≥99.5% (titration), powder, for molecular biology, BioUltra
Recommended Products
grade
for molecular biology
Quality Level
product line
BioUltra
Assay
≥99.5% (T)
form
powder
impurities
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
ign. residue
≤0.1% (as SO4)
loss
≤0.5% loss on drying, 110 °C
pH
5.0-6.5 (25 °C, 1 M in H2O)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
H2O: 1 M at 20 °C, clear, colorless
anion traces
chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg
cation traces
Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg
λ
1 M in H2O
UV absorption
λ: 260 nm Amax: 0.045
λ: 280 nm Amax: 0.035
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
Application
- DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
- RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
- Recapture solution to re-capture RNA exosomes from the glycerol gradient
- ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
- 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors
Other Notes
comparable product
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
54457-BULK-F:
54457-10G-F:
54457-250G-F:
54457-VAR-F:
54457-50G-F:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service