Merck
  • 63493
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63493

Sigma-Aldrich

Hoechst 34580

Trihydrochloride salt, ≥98.0% (HPLC)

Synonym(s):
N,N-Dimethyl-4-[5-(4-methyl-1-piperazinyl)[2,5′-bi-1H-benzimidazol]-2′-yl]benzenamine trihydrochloride, HOE 34580
Empirical Formula (Hill Notation):
C27H29N7 · 3HCl
CAS Number:
Molecular Weight:
560.95
PubChem Substance ID:
NACRES:
NA.32

Quality Level

Assay

≥98.0% (HPLC)

quality

Trihydrochloride salt

solubility

H2O: 1 mg/mL, clear, slightly yellow to deep greenish-yellow

fluorescence

λex 357 nm; λem 490 nm±10 nm in H2O (free dye)

storage temp.

−20°C

SMILES string

Cl.Cl.Cl.CN1CCN(CC1)c2ccc3nc([nH]c3c2)-c4ccc5nc([nH]c5c4)-c6ccc(cc6)N(C)C

InChI

1S/C27H29N7.3ClH/c1-32(2)20-7-4-18(5-8-20)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-9-21(17-25(23)31-27)34-14-12-33(3)13-15-34;;;/h4-11,16-17H,12-15H2,1-3H3,(H,28,30)(H,29,31);3*1H

InChI key

IPMZLKUUQSFUTA-UHFFFAOYSA-N

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Hoechst 34580 Trihydrochloride salt, ≥98.0% (HPLC)

Sigma-Aldrich

63493

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Hoechst 33258 solution 1 mg/mL in H2O, ≥98.0% (HPLC)

Sigma-Aldrich

94403

Hoechst 33258 solution

HTH-01-015 ≥98% (HPLC)

Sigma-Aldrich

SML1446

HTH-01-015

HOE 140 ≥94%

Sigma-Aldrich

H157

HOE 140

assay

≥98.0% (HPLC)

assay

≥98.0% (HPLC)

assay

≥98% (HPLC)

assay

≥94%

quality

Trihydrochloride salt

quality

-

quality

-

quality

-

solubility

H2O: 1 mg/mL, clear, slightly yellow to deep greenish-yellow

solubility

DMF: soluble, H2O: soluble

solubility

DMSO: 5 mg/mL, clear (warmed)

solubility

-

fluorescence

λex 357 nm; λem 490 nm±10 nm in H2O (free dye)

fluorescence

λex 355 nm; λem 465 nm in TE buffer; DNA

fluorescence

-

fluorescence

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

200

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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