77617

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 25:24:1

• CAS Number: 136112-00-0
• MDL number: MFCD00133763
• PubChem Substance ID: 329767815
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• product line: BioUltra
• form: liquid
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH: 7.7-8.3(H₂O-phase, after extraction with H₂O, 1:1)
• density: 1.28 g/mL at 20 °C
• absorption: cut-off at 360 nm
• storage temp.: 2-8°C
• SMILES string: ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1
• InChI: 1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
• InChI key: ZYWFEOZQIUMEGL-UHFFFAOYSA-N

Description

Application

Phenol − chloroform − isoamyl alcohol mixture has been used in nucleic acid extraction in bile duct tissue, Drosophila, chromatin and RNA immunoprecipitation in Caenorhabditis elegans and mitochondrial DNA.

Packaging

100, 100, 500, 500 mL

Physical form

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 25:24:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline

Other Notes

In the extraction of DNA for the generation of DNA minisatellite fingerprints

Safety Information

• Pictograms: GHS05,GHS06,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H301 + H331 - H312 - H314 - H336 - H341 - H351 - H361d - H372 - H373 - H411
• Precautionary Statements: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): 174.2 °F
• Flash Point(C): 79 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves