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CMC0023

Sigma-Aldrich

CONTROLLER SIG10 Chemically Competent Cells

for controlled protein expression

NACRES:
NA.85

grade

for molecular biology

cell transformation

competent cell type: chemically competent
transformation efficiency: >1 × 109 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The CONTROLLER SIG10 Chemically Competent Cells are an E. coli strain optimized for high efficiency transformation.
They are ideal for cloning and propagation of plasmid clones. They give high yield and high quality plasmid DNA due to the endA1 mutation.

Genotype

mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ (ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA Mini-F lacIq1 (GentR)

Features and Benefits

  • Reduce leaky expression: The LacI repressor protein binds to the lacO operator to block transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter will be greatly reduced.
  • High protein yields: Both 10G and BL21(DE3)-based strains are noted for their ability to express high levels of protein. For expression from a T7 promoter please select the BL21(DE3) strain.
This strain also is deficient in proteases, yielding more stable protein preparations. Most other promoters can be used in either of the CONTROLLER strain.
  • Terrific efficiency: The simple, reliable heat-shock procedure for chemical transformation gives excellent results from small amounts of plasmid DNA.

Components

  • CONTROLLER SIG10 chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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