A0810

Endoglycosidase H from Streptomyces plicatus

recombinant, expressed in E. coli, buffered aqueous solution

• Synonym(s): β-N-Acetylglucosaminidase H
• CAS Number: 37278-88-9
• EC Number: 3.2.1.96 (BRENDA, IUBMB)
• MDL number: MFCD00146843
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• conjugate: (N-linked)
• form: buffered aqueous solution
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Endoglycosidase H or endo-β-N-acetylglucosaminidase H is an glycohydrolase. It is produced by Streptomyces plicatus and other Streptomyces species.[1]

Biochem/physiol Actions

Endoglycosidase H is involved in cleaving the N-linked glycans present between the two N-acetylglucosamine (GlcNAc) residues in the core of the glycan chain in high-mannose sugars.[2]

Unit Definition

One unit will release N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per min at 37 °C at pH 5.5.

Physical form

Solution in 20 mM Tris HCl, pH 7.5, containing 50 mM NaCl, 1 mM EDTA

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves