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A3311

Sigma-Aldrich

Bovine Serum Albumin

powder, BioXtra

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Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

biological source

bovine

Quality Level

product line

BioXtra

Assay

≥98% (agarose gel electrophoresis)

form

powder

mol wt

~66 kDa

packaging

poly bottle of

origin

USA origin

technique(s)

cell culture | embryo: suitable

loss

≤5%

pH

6.5-7.5

solubility

water: soluble (40 mg/ml)

shipped in

ambient

storage temp.

2-8°C

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This Item
A1470A7638A4612
Bovine Serum Albumin powder, BioXtra

Sigma-Aldrich

A3311

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, low endotoxin, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)

Sigma-Aldrich

A1470

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

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A7638

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, Essentially fatty acid free

Sigma-Aldrich

A4612

Bovine Serum Albumin

assay

≥98% (agarose gel electrophoresis)

assay

≥98% (agarose gel electrophoresis)

assay

≥99% (agarose gel electrophoresis)

assay

≥98% (agarose gel electrophoresis)

form

powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

origin

USA origin

origin

USA origin

origin

USA origin

origin

New Zealand origin

Application

This albumin has been qualified for use in media designed to support in vitro applications such as mouse blastocyst and morulae culture.

Biochem/physiol Actions

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Nature communications, 9(1), 4234-4234 (2018-10-14)
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Articles

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Related Content

Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.

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