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A3574

Sigma-Aldrich

Acrylamide/Bis-acrylamide solution

30%, BioReagent, suitable for electrophoresis, 29:1

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Synonym(s):
30% Acrylamide/Bisacrylamide 29:1, Polyacrylamide solution
MDL number:
UNSPSC Code:
41105319
PubChem Substance ID:
NACRES:
NA.25

product name

Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 29:1

sterility

0.2 μm filtered

Quality Level

product line

BioReagent

form

liquid

feed ratio

29:1

technique(s)

electrophoresis: suitable

suitability

suitable for electrophoresis

foreign activity

Protease, none detected

storage temp.

2-8°C
2-8°C

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 4% stacking gel and 10% resolving gel during sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis. It has also been used as a precursor for polyacrylamide lid fabrication by photopatterning methods.
These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

PRTR

Class I Designated Chemical Substances

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

A3574-100ML:4548173185514
A3574-5X100ML:4548173185521
A3574-PH:
A3574-5L:4548173224534
A3574-100ML-PW:
A3574-VAR:
A3574PROC:
A3574-5L-PW:
A3574-BULK:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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