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A4414

Sigma-Aldrich

Albumin from porcine serum

lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

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CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

biological source

Porcine

Quality Level

Assay

≥99% (agarose gel electrophoresis)

form

lyophilized powder

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

Porcine ... ALB(396960)

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This Item
A2764A1830A3139
Albumin from porcine serum lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

A4414

Albumin from porcine serum

Albumin from porcine serum lyophilized powder, 96-99% (agarose gel electrophoresis)

A2764

Albumin from porcine serum

Albumin from porcine serum lyophilized powder, ≥98% (agarose gel electrophoresis)

A1830

Albumin from porcine serum

Albumin from mouse serum lyophilized powder, ≥96% (agarose gel electrophoresis)

A3139

Albumin from mouse serum

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable, electrophoresis: suitable

assay

≥99% (agarose gel electrophoresis)

assay

96-99% (agarose gel electrophoresis)

assay

≥98% (agarose gel electrophoresis)

assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Gene Information

Porcine ... ALB(396960)

Gene Information

Porcine ... ALB(396960)

Gene Information

Porcine ... ALB(396960)

Gene Information

mouse ... ALB(11657)

General description

Albumin is present abundantly in serum. Porcine serum albumin (PSA) comprises a flexible C-terminal region compared to other albumins.

Application

Albumin from porcine serum has been used:
  • as a non-hazardous protein to test its effect on T84 polarized intestinal monolayers and Caco2 polarized intestinal monolayers barrier integrity in trans-Epithelial Electrical Resistance (TEER) assay
  • to test its effect on the human intestinal epithelial cell lines
  • to test its interaction with the biodegradable ester-functionalized gemini surfactant 16-E2-16 using ultraviolet (UV)-visible and circular dichroism (CD) spectroscopy measurements

Biochem/physiol Actions

Albumin binds to many blood associated proteins. Porcine serum albumin (PSA) is regarded as a major allergen. PSA is used in the immunosensors, molecularly imprinted nanogels (MIP-NGs) and enzyme-linked immunosorbent assay (ELISA) protein-based detection for assessing contamination, especially in meat products.

Preparation Note

Prepared from albumin (A2764).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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An ester-functionalized cationic gemini surfactant mediated structural transitions of porcine serum albumin (PSA) via binding interaction
Akram M, et al.
Colloids and Surfaces. A, Physicochemical and Engineering Aspects, 516, 245-253 (2017)
Sung-Sil Moon et al.
Korean journal for food science of animal resources, 34(3), 355-361 (2014-01-01)
This study was conducted to evaluate the changes in porcine serum albumin (PSA), a major allergen, which occur when raw pork ham is marinated with kiwi or pineapple juice, and/or when the ham is pan broiled at 300℃ for 4
Chehasan Cheubong et al.
Analytical chemistry, 92(9), 6401-6407 (2020-04-14)
Accurate, simple, and valuable analytical methods for detection of food contamination are rapidly expanding to evaluate the validity of food product quality because of ethnic considerations and food safety. Herein molecularly imprinted nanogels (MIP-NGs), capable of porcine serum albumin (PSA)
Bryan P Hurley et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 92, 75-87 (2016-04-10)
Human intestinal epithelial cell lines (T84, Caco-2, and HCT-8) grown on permeable Transwell™ filters serve as models of the gastrointestinal barrier. In this study, this in vitro model system was evaluated for effectiveness at distinguishing between hazardous and non-hazardous proteins. Indicators
C Zimmermann et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 115, 451-459 (2018-04-04)
Recent studies suggest that human derived intestinal epithelial cells (IECs) cultured as polarized monolayers on Transwell® filters may respond differently when exposed to hazardous and non-hazardous proteins. This experimental platform was based on apical exposure of IEC monolayers to test

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