A9511

Albumin from human serum

lyophilized powder, ≥97% (agarose gel electrophoresis)

• Synonym(s): HSA
• CAS Number: 70024-90-7
• EC Number: 274-272-6
• MDL number: MFCD00081418
• eCl@ss: 42010201
• NACRES: NA.25

Properties

• biological source: human
• Quality Level: 300
• Assay: ≥97% (agarose gel electrophoresis)
• form: lyophilized powder
• mol wt: monomer calculated mol wt 66478 Da
• technique(s): ELISA: suitable; tissue culture: suitable; western blot: suitable
• impurities: HIV I and HIVII, HCV and HBsAg, tested negative
• solubility: H₂O: soluble 50 mg/mL
• UniProt accession no.: P02768
• storage temp.: 2-8°C
• InChI: 1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11
• InChI key: QYSGYZVSCZSLHT-UHFFFAOYSA-N
• Gene Information: human ... ALB(213)

Description

General description

Albumin is the most copious protein in blood plasma. Liver produces human albumin.

Human albumin possess a molecular weight of 67 kDa.

Application

Albumin from human serum has been used:

• to standardize the column for molecular-exclusion chromatography
• to prepare the tobacco mosaic virus (TMV)-poly ethylene glycol (PEG8)-serum albumin (SA) particles
• to analyze its modifications after oxidation using a Fenton system

Albumin-bound fluorescence was used in serum of patients with chronic renal failure.

Biochem/physiol Actions

Albumin turnover is seen in infants with iron deficiency anemia. Serum albumin is a reliable prognostic indicator in liver disease. Oxidative modification of albumin is seen in advanced liver disease.

Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones, and is vital in regulating the colloidal osmotic pressures of blood. Albumin is also seen to bind to exogenous substances, particularly drugs (e.g., ibuprofen, warfarin), and strongly influence their pharmacokinetics. Oxidative stress leading to changes in the redox state of albumin has widely varied effects on its physiological function.

Preparation Note

Prepared using method IV of Cohn, E.J., et al., J. Am. Chem. Soc., 69, 1753 (1947).

Other Notes

View more information on human serum albumin.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves