Agarose is a polysaccharide made up of alternating ?-D-galactose and 3,6-anhydro-L-galactose units of agarobiose in its chemical structure. The molecular weight is variable and has not been determined. Please see the link below for additional information, including an agarose selection guide:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/181/864/a9539pis.pdf
A9539
Agarose
BioReagent, Molecular Biology, low EEO
Agarose
1 of 2 reviewers received a sample product or took part in a promotion
Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE
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10 G
¥5,880
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¥11,100
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¥19,800
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¥30,300
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¥66,600
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¥109,000
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10 G
¥5,880
25 G
¥11,100
50 G
¥19,800
100 G
¥30,300
250 G
¥66,600
500 G
¥109,000
About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41105317
PubChem Substance ID:
NACRES:
NA.25
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biological source
algae (marine)
Quality Level
grade
Molecular Biology
product line
BioReagent
form
powder
technique(s)
electrophoresis: suitable
impurities
≤10% moisture content
EEO
0.09-0.13
transition temp
gel point 36 °C ±1.5 °C (1.5% gel)
gel strength
≥1200 g/cm2 (1% gel)
anion traces
sulfate (SO42-): ≤0.15%
suitability
suitable for electrophoresis
suitable for molecular biology
foreign activity
DNase, RNase, none detected
SMILES string
O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
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1 of 4
This Item | A0169 | A2576 | A0576 |
|---|---|---|---|
| EEO 0.09-0.13 | EEO 0.09-0.13 | EEO ≤0.12 | EEO ≤0.12 |
| form powder | form powder | form powder | form powder |
| gel strength ≥1200 g/cm2 (1% gel) | gel strength ≥1200 g/cm2 (1% gel) | gel strength ≥300 g/cm2 (1.5% gel) | gel strength ≥1800 g/cm2 (1% gel), ≥3200 g/cm2 (1.5% gel) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line BioReagent | product line - | product line - | product line - |
| grade for molecular biology | grade - | grade Molecular Biology | grade - |
General description
Agarose is a natural polysaccharide isolated from the seaweed genera Gelidium and Gracilaria. Structurally, it is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units.[1]
Application
Agarose has been used:
- in gel electrophoresis to analyze the integrity of DNA[2]
- to prepare hydrocolloid gels and study the structural influence of gels on the release of carbohydrates[3]
- in gel electrophoresis to analyze the integrity of RNA[4]
- it is suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID)
Biochem/physiol Actions
Agarose serves as a gelling agent and is extensively used in molecular biology research to separate and analyze nucleic acids by gel electrophoresis or blotting (Northern or Southern). During gelation, agarose polymers combine non-covalently resulting in a network of bundles whose pore sizes decide a gel′s molecular sieving properties. The type and the concentration of agarose influence the rate of migration of a DNA molecule through a gel.[1] However, agarose also has attractive features that bring about a strong interest in its usage in biological applications. It is known to mimic the extracellular matrix which makes it an excellent biomaterial for tissue engineering applications. Due to its great water uptake capability agarose is suitable for cell-encapsulation. Agarose allows regulated permeation for oxygen and nutrients and is useful in cell growth, differentiation, and proliferation. Agarose is used in controlled/localized drug delivery and regenerative medicine, such as neurogenesis, angiogenesis, spermatogenesis, cartilage formation, bone regeneration, wound healing, and artificial pancreas.[5]
Features and Benefits
- Biocompatible
- Agarose gels have larger pore sizes than polyacrylamide gels at low concentrations
- Unlike polyacrylamide, the consistency of the gels is more solid (but also less elastic)
- Possesses low ethidium bromide and SYBR Green background staining
Analysis Note
The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
80628:
A9539-100G:
A9539-VAR:
A9539-10G:
A9539-6X100G:
A9539-25G:
A9539-BULK:
A9539-250G:
A9539-50G:
A9539-500G:
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I would like to know the molecular weight of this agarose.
1 answer-
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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Good afternoon, The specification of this product indicates that the amount of sulfate is lower or equal to 15%. Could you please specify what the 15% refers to? mass of sulfate groups per total mass of the polymer? Best regards
1 answer-
This product contains trace amounts of sulfate. The maximum amount allowable is 0.15% by weight.
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