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A9647

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, pH 7, ≥98%

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Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

biological source

bovine

Quality Level

Assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

origin

USA origin

technique(s)

immunocytochemistry: suitable

impurities

≤5.0% Loss on drying

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

foreign activity

BT virus, none detected
VSV virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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This Item
A4503A3912A3803
Bovine Serum Albumin heat shock fraction, pH 7, ≥98%

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A9647

Bovine Serum Albumin

Bovine Serum Albumin cold ethanol fraction, pH 5.2, ≥96%

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A4503

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, pH 5.2, ≥96%

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A3912

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, lyophilized powder, essentially fatty acid free, ≥98% (agarose gel electrophoresis)

Sigma-Aldrich

A3803

Bovine Serum Albumin

assay

≥98%

assay

≥96%

assay

≥96%

assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

purified by

heat shock fractionation

purified by

cold ethanol fractionation

purified by

heat shock fractionation

purified by

heat shock fractionation

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

General description

Bovine serum albumin is a protein whose key biological function is to regulate the colloidal osmotic pressure of blood. BSA is widely used in various studies and applications. Made up of 583 amino acids, BSA is a water-soluble protein with a calculated molecular weight of 66,430 Da. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

Application

Bovine Serum Albumin is used for many applications, including the following applications:
  • BSA has been used in cell culture, e.g. as a supplement in media prepared for culturing of oligodendrocytes.
  • BSA has been used for the dilution of primary antibody solution in immunohistochemistry procedures.
  • BSA has been used as a standard in the Bradford method of protein quantification.
  • BSA has been used in blocking and permeabilization buffer for immunofluorescence studies.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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