Merck
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C-12281

Sigma-Aldrich

Human Pulmonary Microvascular Endothelial Cells (HPMEC)

500,000 cryopreserved cells

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Synonym(s):
HPMEC cells

biological source

human lung

packaging

pkg of 500,000 cells

morphology

( endothelial)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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Human Pulmonary Microvascular Endothelial Cells (HPMEC) 500,000 cryopreserved cells

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Human Pulmonary Microvascular Endothelial Cells (HPMEC)

Human Pulmonary Artery Endothelial Cells (HPAEC) 500,000 cryopreserved cells

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Human Pulmonary Microvascular Endothelial Cells (HPMEC) 1 million cells per pellet

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Human Pulmonary Microvascular Endothelial Cells (HPMEC)

Human Cardiac Microvascular Endothelial Cells (HCMEC) 500,000 cryopreserved cells

Sigma-Aldrich

C-12285

Human Cardiac Microvascular Endothelial Cells (HCMEC)

biological source

human lung

biological source

human pulmonary artery

biological source

human lung

biological source

human heart (ventricles)

packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 500,000 cells

morphology

( endothelial)

morphology

( endothelial)

morphology

(endothelial)

morphology

( endothelial)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−20°C

storage temp.

−196°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Lungs

Application

Primary Human Pulmonary Microvascular Endothelial Cells (HPMEC) are isolated from the lung from a single donor and are provided in a cryopreserved format. Since lung tissue contains blood and lymphatic capillaries, HPMEC comprise Blood and Lymphatic Microvascular Endothelial Cells. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and von Willebrand factor and negative for smooth muscle alpha-actin.The lung has a vast endothelial surface area, which is essential for the exchange of gases. HPMEC are most appropriate for studying human lung diseases.

Quality

Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested forthe absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

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