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C2978

Sigma-Aldrich

CelLytic™ M

Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.

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Synonym(s):
Whole-cell protein extraction solution
NACRES:
NA.56

Quality Level

200

form

solution

storage temp.

room temp

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This Item
CE0050CB050004719964001
CelLytic™ M Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.

Sigma-Aldrich

C2978

CelLytic™ M

CelLytic™ MEM Protein Extraction Kit

Sigma-Aldrich

CE0050

CelLytic™ MEM Protein Extraction Kit

Sigma-Aldrich

Sigma-Aldrich

CB0500

CelLytic™ B Plus Kit

cOmplete™ Lysis-M EDTA-free

Roche

04719964001

cOmplete™ Lysis-M EDTA-free

storage temp.

room temp

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

-

General description

CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.

Features and Benefits

  • Efficient: Up to 50% more efficient than freeze thaw, sonication and other products
  • Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays
  • Convenient: Ready-to-use reagent requires no scraping from culture plates
  • Fast: Rapid cell lysis at room temperature.

Quantity

CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).

Analysis Note

Use 125 μl CelLytic M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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