Merck
All Photos(3)

C6158

Sigma-Aldrich

Crystal Violet

ACS reagent, ≥90.0% anhydrous basis

Synonym(s):
Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
Linear Formula:
C25H30N3Cl
CAS Number:
Molecular Weight:
407.98
Colour Index Number:
42555
Beilstein:
3580948
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

ACS reagent

Quality Level

assay

≥90.0% anhydrous basis

form

powder

loss

≤7.5% loss on drying

mp

205 °C (dec.) (lit.)

solubility

water: soluble 50 g/L at 27 °C

density

1.19 g/cm3 at 20 °C

absorption

passes test

suitability

passes test for sensitivity as indicator

antibiotic activity spectrum

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

Mode of action

cell membrane | interferes
enzyme | inhibits

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

Application

Crystal violet has been used to determine cell viability. It has been used in the staining of cells for the quantification of cellular migration.

Packaging

50, 100 g in glass bottle

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal violet helps in colorimetric measurement of cell viability.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service