Merck
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C6534

Sigma-Aldrich

Anti-Chicken Egg Albumin antibody produced in rabbit

whole antiserum

Synonym(s):
Anti-Albumin, chicken egg
MDL number:
NACRES:
NA.46

biological source

rabbit

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

chicken

technique(s)

indirect ELISA: 1:80,000
quantitative precipitin assay: 3-5 mg/mL

shipped in

dry ice

storage temp.

−20°C

Gene Information

chicken ... Alb(396197)

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biological source

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conjugate

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antibody form

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antibody form

whole antiserum

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OVA-14, monoclonal

clone

OVA-14, monoclonal

clone

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species reactivity

chicken

species reactivity

chicken, turkey

species reactivity

chicken, turkey

species reactivity

-

technique(s)

indirect ELISA: 1:80,000, quantitative precipitin assay: 3-5 mg/mL

technique(s)

competitive inhibition ELISA: suitable, dot blot: suitable, immunoblotting: suitable, indirect ELISA: 0.1-0.2 μg/mL using 10 μg/mL Albumin from chicken egg white (A5503) for coating.

technique(s)

dot blot: suitable, indirect ELISA: 1:10,000 using chicken egg albumin (ovalbumin), western blot: suitable

technique(s)

indirect ELISA: 1:40,000, quantitative precipitin assay: 2.8 mg/mL

General description

Chicken/hen′s egg albumin, also known as ovalbumin (OVA), is the most abundant egg white protein. It is surrounded by B-cell epitopes, which are bound by specific IgE antibodies. In addition, it also contains CD4+ T cell epitopes. Ovalbumin is a monomeric protein with a molecular mass of 45000 Da.

Application

Anti-Chicken Egg Albumin antibody produced in rabbit has been used in:
  • quantitative precipitin assay
  • indirect enzyme linked immunoassay (ELISA)
  • development of nonimmune rabbit antiserum against chicken ovalbumin

Biochem/physiol Actions

Chicken/hen′s egg albumin or ovalbumin (OVA) is a major allergen found in egg white and is widely used in allergy research. It has been widely used to determine the characteristics of class II major histocompatibility complex (MHC)-peptide binding and T-cell activation.

Preparation Note

treated to remove lipoproteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Poisonous substance


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