C9249

CryoSOfree^™ DMSO-free Cryopreservation Medium

Name: CryoSOfree<SUP>™</SUP> DMSO-free Cryopreservation Medium
Brand: SIGMA
Price: 79800 JPY
Availability: InStock

Synonym(s):

cell freezing medium

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100 ML

¥79,800

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

Key Documents

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form

liquid

Quality Level

200

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

cell culture | mammalian: suitable
cryopreservation: suitable

greener alternative category

, Aligned

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Animal Component Free Cell Culture Reagents

Cell Culture & Analysis

Cell Culture Media & Buffers

Cell Culture Supplements & Reagents

Cell Freezing Reagents

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This Item	C6295	C6164	C2999
Sigma-Aldrich C9249 CryoSOfree^™ DMSO-free Cryopreservation Medium	Sigma-Aldrich C6295 Cell Freezing Medium-DMSO Serum free 1x	Sigma-Aldrich C6164 Cell Freezing Medium-DMSO 1×	Sigma-Aldrich C2999 CryoStor^® cell cryopreservation media
form liquid	form liquid	form liquid	form liquid
technique(s) cell culture \| mammalian: suitable, cryopreservation: suitable	technique(s) cell culture \| mammalian: suitable, cryopreservation: suitable	technique(s) cell culture \| mammalian: suitable, cryopreservation: suitable	technique(s) cell culture \| mammalian: suitable, cryopreservation: suitable
shipped in wet ice	shipped in dry ice	shipped in dry ice	shipped in ambient
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
greener alternative product characteristics Designing Safer Chemicals Learn more about the Principles of Green Chemistry.	greener alternative product characteristics -	greener alternative product characteristics -	greener alternative product characteristics -

General description

Cultured cells in a suspension can be cryopreserved with minimal damage from freezing and thawing steps. CryoSOfree^™ DMSO-free cryopreservation medium preserves them with high viability and physiological activity. This medium is recommended for the preservation of stem cells, hepatocytes, tissue sample and a wide variety of cell types and established cell lines. CryoSOfree^™ DMSO-free cryopreservation medium is a proprietary formulation, containing no DMSO nor animal proteins. It is ready-to-use and complete with no additives required.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of DMSO for cryopreservation of cell cultures. For more information see here.

Application

CryoSOfree is a chemically defined DMSO-free cryopreservation medium. It is animal component free and has been shown[1][2] to preserve the potential of stem cell for differentiation.

Features and Benefits

Preserves pluripotency of stem cells
Low cytotoxicity
Chemically defined, serum free, protein free
Ready to use

The following cell lines have been recovered at ≥90% following cryopreservation with CryoSOfree:
L929, KB, MC3T3, B16F1, Colon26, CaCO2, HT1080, MG63

Preparation Note

1 ml of CyroSOfree is sufficient for 5x10⁵- 5x10⁶ cells.

Legal Information

CryoSOfree is a trademark of Sigma-Aldrich Co. LLC

Pictograms

GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319

Precautionary Statements

P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

C9249-VAR:
C9249-PH:
C9249-BULK:
C9249-100ML-PW:
C9249-100ML:4548173965307
C9249PROC:

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Certificates of Analysis (COA)

Lot/Batch Number

0000488461

0000448404

0000427940

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Polyampholytes as cryoprotective agents for mammalian cell cryopreservation.

Kazuaki Matsumura et al.

Cell transplantation, 19(6), 691-699 (2010-06-08)

Cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol, ethylene glycol, and propylene glycol have been used for the cryopreservation of cells and tissues. DMSO is the most effective CPA but shows high cytotoxicity and can effect differentiation. ɛ-poly-L-lysine (PLL)

Polyampholytes as low toxic efficient cryoprotective agents with antifreeze protein properties.

Kazuaki Matsumura et al.

Biomaterials, 30(27), 4842-4849 (2009-06-12)

Dimethyl sulfoxide (DMSO) has been used for several decades as the most efficient cryoprotective agent (CPA) for many types of cells and tissues in spite of its cytotoxicity and its effects on differentiation. Here we report that polyampholytes with an

Ex vivo SARS-CoV-2 infection of human lung reveals heterogeneous host defense and therapeutic responses.

Matthew A Schaller et al.

JCI insight, 6(18) (2021-08-07)

Cell lines are the mainstay in understanding the biology of COVID-19 infection but do not recapitulate many of the complexities of human infection. The use of human lung tissue is one solution for the study of such novel respiratory pathogens.

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CryoSOfree™ DMSO-free Cryopreservation Medium