Merck
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CMC0016

Sigma-Aldrich

BL21(DE3) Electrocompetent Cells

for protein expression

Synonym(s):
BL21 strain
NACRES:
NA.85

grade

for molecular biology

cell transformation

competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Features and Benefits

The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 109 cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment

Components

  • BL21(DE3) electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Storage Class Code

10 - Combustible liquids

WGK

WGK 3


Regulatory Listings

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