Merck
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D8900

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

All Photos(1)
Synonym(s):
DMEM Hams F12, DME/F-12, 1:1 mixture
MDL number:
MFCD00217417

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Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:

  • as a component of the culture medium for endometrial explants in vitro culture
  • to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
  • to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein

Packaging

1, 10, 50 L

Quantity

Formulated to contain 15.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Certificate of Analysis

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Certificate of Origin

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