E9762

Endoglycosidase F1

Cleaves within N-linked glycans, suitable for Mass Spectrometry, from Elizabethkingia miricola, recombinant, expressed in E. coli

• Synonym(s): Endo F1, Endo-β-N-acetylglucosaminidase F1, Endoglycosidase F1 from Chryseobacterium meningosepticum, Endoglycosidase F1 from Elizabethkingia meningoseptica, Endoglycosidase F1 from Flavobacterium meningosepticum
• EC Number: 3.2.1.96 (BRENDA, IUBMB)
• MDL number: MFCD00146843
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• Product Name: Endoglycosidase F1 from Elizabethkingia miricola, recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution
• recombinant: expressed in E. coli
• Quality Level: 200
• conjugate: (N-linked)
• form: buffered aqueous solution
• specific activity: ≥16 U/mg
• mol wt: 32 kDa
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Endoglycosidase F1 from Elizabethkingia miricola is a glycan specific enzyme.[1]

Application

Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides.

Endoglycosidase F1 from Elizabethkingia miricola has been used to remove binding of human hemochromatosis protein (HFE) to cation independent mannose-6-phosphate receptor (CI-MPR)[2] and to glycosylate fucosylated N-glycopeptides.[3]

Biochem/physiol Actions

Endoglycosidase F1 from Elizabethkingia miricola plays an important role in cleavage of glycan structures from the protein by cleaving between the two N-acetylglucosamine residues of the chitobiose core. It mediates high mannose and hybrid oligosaccharides cleavage.[4]

Packaging

Supplied with 5× Reaction Buffer, 250 mM NaH₂PO₄, pH 5.5.

Unit Definition

One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5.

Physical form

Aseptically filled solution in 20 mM Tris-HCl pH 7.5

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves