EDEGLY

Enzymatic Protein Deglycosylation Kit

• Synonym(s): Glycosylation Release Kit
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• conjugate: (N-linked); (O-linked)
• Quality Level: 200
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Enzymatic protein deglycosylation kit contains all the enzymes and reagents required for the complete removal of N-linked and simple O-linked carbohydrates from glycoproteins. This kit is employed to cleave the complex Core 2 O-linked carbohydrates including those containing polylactosamine.

Application

Enzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.[1][2][3]

Enzymatic protein deglycosylation kit has been used in:

• protein deglycosylation[4]
• deglycosylation of tissue factor pathway inhibitor (TFPI) α and β[5]
• deglycosylation of native exo-β-(1,3)-glucanase EXG1[6]

Features and Benefits

Enzymatic protein deglycosylation kit has the following features:

• two or more mg of glycoprotein is deglycosylated
• single reaction at neutral pH
• native & denaturing procedures
• no degradation of protein
• O-linked sugars containing polysialic acid are eliminated
• control glycoprotein provided

Kit Components Only

• PNGase F 1 mL/vial
• O-Glycosidase 20 μL
• α-2(3,6,8,9)-Neuraminidase 20 μL
• Fetuin Control .5 mg
• 5× Reaction Buffer .2 mL
• Denaturation Solution .1 mL
• Triton^™ X-100 .1 mL
β-1→4-Galactosidase, positionally specific 20 μL
β-N-Acetylglucosaminidase 20 μL

See All (9)

Safety Information

• Pictograms: GHS08,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H312,H315,H317,H318,H334,H361f,H410
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable