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F0895

Sigma-Aldrich

FIBRONECTIN FROM HUMAN PLASMA

liquid, 0.1% (Solution), BioReagent, suitable for cell culture

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Synonym(s):
Fibronectin
MDL number:
MFCD00131062
eCl@ss:
42020141
NACRES:
NA.75

biological source

human plasma

Quality Level

200

sterility

sterile

product line

BioReagent

form

liquid

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

concentration

0.1% (Solution)

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

solubility

saline: 0.05 M (Tris-buffered)

UniProt accession no.

P02751

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

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Fibronectin human plasma liquid, 0.1% (Solution), BioReagent, suitable for cell culture

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form

liquid

form

lyophilized powder

form

lyophilized powder

form

solution

packaging

pkg of 1 mg, pkg of 2 mg, pkg of 5 mg

packaging

pkg of 0.5 mg

packaging

pkg of 0.5 mg

packaging

pkg of 1 mg, pkg of 2 mg, pkg of 5 mg

concentration

0.1% (Solution)

concentration

-

concentration

-

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

solubility

saline: 0.05 M (Tris-buffered)

solubility

water: soluble >0.5 mg/mL, clear to slightly hazy, colorless

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

solubility

-

General description

Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. The FN1 gene mapped to human chromosome 2q35.

Application

Fibronectin human plasma has been used in the determination of H2O2 consumption rate. It has also been used in coating the Sylgard® models for cell culture. It has also been used in cell adhesion and to directly pattern the protein onto the polymeric film.

Biochem/physiol Actions

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis.

Preparation Note

This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Legal Information

Sylgard is a registered trademark of The Dow Chemical Company or an affiliated company of Dow

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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25G
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Protocols

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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