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F1056

Sigma-Aldrich

Fibronectin human plasma

lyophilized powder, BioReagent, suitable for cell culture, ≥95% (SDS-PAGE)

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Synonym(s):
Fibronectin
EC Number:
MDL number:
NACRES:
NA.75

biological source

human plasma

Quality Level

sterility

sterile

product line

BioReagent

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

solubility

H2O: 1 mg/mL at 37 °C

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FN1(2335)

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This Item
F2006FC010F1903
sterility

sterile

sterility

sterile

sterility

sterile; sterile-filtered

sterility

-

assay

≥95% (SDS-PAGE)

assay

≥85% (SDS-PAGE)

assay

~95% (SDS-PAGE)

assay

-

form

lyophilized powder

form

lyophilized powder

form

liquid

form

lyophilized

packaging

pkg of 1 mg, pkg of 2 mg, pkg of 5 mg

packaging

pkg of 5 x 5 mg, pkg of 1 mg, pkg of 2 mg, pkg of 5 mg

packaging

-

packaging

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Caution

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

Preparation Note

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocols

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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